GOGAT 抗体
The 兔 多克隆 anti-GOGAT antibody (ABIN3155732) specifically detects GOGAT in WB.
The antibody is reactive with Arabidopsis thaliana, Green bean, Miscanthus giganteus, Nicotiana tabacum, Oryza sativa, Populus tremula, Spartina, Tomato 和 Zea mays samples.
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产品编号 ABIN3155732
发货至:
中国
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Quick Overview for GOGAT 抗体 (ABIN3155732)
抗原
GOGAT
(Glutamine Oxoglutarate Aminotransferase (GOGAT))
适用
Arabidopsis thaliana, Green bean, Miscanthus giganteus, Nicotiana tabacum, Oryza sativa, Populus tremula, Spartina, Tomato, Zea mays
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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原理
- Anti-GOGAT | Glutamine oxoglutarate aminotransferase
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交叉反应 （详细）
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Arabidopsis thaliana, Miscanthus giganteus, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Populus tremula, Solanum lycopersicum, Spartina sp., Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Arthrospira platensis PCC 7345,Beta vulgaris,Brachypodium distachyon,Burkholderia glumae,Camellia sinensis,Chloroflexi bacterium,Chlamydonas reinhardtii,Gracilaria tenustipitata,Cyanobacteria,Crocosphaera watsonii WH 8502,Cyanidioschyzon merolae (strain 10D),Galdieria sulphuraria,Glycine max,Helicosporidium sp. ATCC 50920,Hydrogenobaculum sp. HO,Lactococcus lactis subsp. lactis bv. diacetylactis str. TIFN2,Leptospira interrogans serovar Bataviae str. HAI135,Leptolyngbya boryana,Medicago trucutula,Microcystis panniformis,Monoraphidium neglectum,Morus notabilis,Nannochloropsis gaditana,Nicotiana tabacum,Ostreococcus lucimarinus,Physcomitrium patens subsp. patens,Porphyra purpurea,Sutterella wadsworthensis,Sulfurihydrogenibium yellowstonense,Veillonella atypica
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纯化方法
- Serum
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免疫原
- KLH-conjugated synthetic peptide well conserved in known GOGAT sequences from different species including Arabidopsis thaliana Fd-GOGAT 1 Q9ZNZ7
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应用备注
- 1 : 1000 (WB)
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说明
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A 40 kDa band present in A. thaliana sample is not competed away during antibody neutralization assay. In this assay free peptide used for antibody production is incubated together with anti-GOGAT antibodies. Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- GOGAT (Glutamine Oxoglutarate Aminotransferase (GOGAT))
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背景
- Glutamine oxoglutarate aminotransferase (abbreviated as GOGAT) is an enzyme involved in synthesis of glutamate from glutamine and alpha-ketoglutarate. GOGAT has two forms in plants: ferredoxin-dependent GOGAT (Fd-GOGAT) and NADH-dependent GOGAT (NADH-GOGAT). 95 % of GOGAT found in plants is the Fd-GOGAT type. Fd-GOGAT is encoded by two genes, glu1 and glu2 found on chromosomes 5 and 2 respectively (in Arabidopsis). Fd-GOGAT (both forms) is highly conserved among plants, red algae, and cyanobacteria.
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分子量
- 177 | 170-180 kDa depending upon the species
抗原
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