(1 reference)-
- 抗原
- EXOS (ExoS Gene Product (EXOS))
- 抗原表位
- cleaved
- 适用
- Pseudomonas aeruginosa
- 宿主
-
小鸡
- 克隆类型
- 多克隆
- 应用范围
- Western Blotting (WB)
- 交叉反应 （详细）
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- 预测反应
- Pseudomonas aeruginosa only
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 48 kDa
- 纯化方法
- affinity purified
- 免疫原
- amino acids 366 to 453 of PA3841 of ADP-rbosylating enzyme - Exoenzyme S overexpressed in a GST fusion. Afterwards cleaved with a help of trombin and separated on a polyacrylamide gel. Gel piece has been used for immunizations.
- 亚型
- IgY
-
- 应用备注
- 1: 5000 (WB)
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- PBS pH 8.0+ 0.02 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Make aliquots to avoid repreated freeze-thaw cycles and working with a stock. - 储存条件
- 4 °C
- 储存方法
- store at 4°C, make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
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-
: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, (2012) (PubMed).
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: "The Role of Type III Secretion System and Lens Material on the Adhesion of Pseudomonas aeruginosa to Contact Lenses." in: Investigative ophthalmology & visual science, (2012) (PubMed).
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- 抗原
- EXOS (ExoS Gene Product (EXOS))
- 别名
- Exoenzyme S (ExoS)
- 背景
- Exoenzyme S (ExoS) is a toxin directly translocated into eukaryotic cells by the type III secretory process of Pseudomonas aeruginosa. It is a bi-functional toxin and contain an N-terminal Rho GAP domain that disrupts the actin cytoskeleton and interference of phagocytosis. The C-terminal of ExoS contains an ADP-ribosyltransferase domain, which elicits a cytotoxic phenotype in cultured cells.
- 分子量
- 48 kDa
- UniProt
- Q51449
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