CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays
ChIC, CUT&RUN
20 X
(5 references)
产品编号 ABIN6950951
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for CUTANA™ pAG-MNase for ChIC/CUT&RUN Assays (ABIN6950951)
抗原
Nuclease
应用范围
Chromatin Immunocleavage (ChIC), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
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宿主
- 大肠杆菌（E. Coli）
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原理
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This construct of Protein A and G fused to Micrococcal Nuclease is useful in performing CUT&RUN.
The pAG-MNase fusion enzyme may be used in conjunction with any of our CUT&RUN Product Sets.
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品牌
- CUTANA™
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产品特性
- Recombinantly produced in E. coli, CUTANA™ pAGMNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN). CUTANA pAG-MNase contains a C-terminal 6xHis epitope tag.
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应用备注
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This product is sufficient to perform 50/250 CUT&RUN reactions.
Recommended use: 2.5 μL of the supplied enzyme into a 50 μL CUT&RUN reaction (20X dilution). -
说明
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CUTANA pAG-MNase, the essential reagent for ChIC/CUT&RUN workflows:
- First-in-class commercial product for ChIC/CUT&RUN assays
- Optimized fusion of Proteins A and G with Micrococcal Nuclease (pAG-MNase) enables direct compatibility with a broad range of antibody isotypes
- 50 and 250 reaction pack sizes, enabling greater experimental throughput
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限制
- 仅限研究用
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浓度
- 20 X
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缓冲液
- Provided as a 20X stock in 10 mM Tris HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, and 50 % glycerol.
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注意事项
- Avoid freeze-thaw cycles
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of receipt. The protein is not subject to freeze/thaw under these conditions.
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有效期
- 12 months
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: "A distal enhancer with ETV4 binding is critical for UCP1 expression and thermogenesis in brown fat." in: Genes & development, Vol. 39, Issue 13-14, pp. 808-825, (2025) (PubMed).
: "Improved CUT&RUN chromatin profiling tools." in: eLife, Vol. 8, (2019) (PubMed).
: "Targeted in situ genome-wide profiling with high efficiency for low cell numbers." in: Nature protocols, Vol. 13, Issue 5, pp. 1006-1019, (2019) (PubMed).
: "An efficient targeted nuclease strategy for high-resolution mapping of DNA binding sites." in: eLife, Vol. 6, (2018) (PubMed).
: "ChIC and ChEC; genomic mapping of chromatin proteins." in: Molecular cell, Vol. 16, Issue 1, pp. 147-57, (2004) (PubMed).
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: "A distal enhancer with ETV4 binding is critical for UCP1 expression and thermogenesis in brown fat." in: Genes & development, Vol. 39, Issue 13-14, pp. 808-825, (2025) (PubMed).
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- Nuclease
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分子量
- 44 kDa
抗原
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