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Proteinase K (Tritirachium album limber)

产品编号 ABIN5706434
发货至: 中国
  • 纯化方法
    A lyophilized powder. Chromatographically purified to remove DNase and RNase activity
    A280 at 1 mg/ml = 0.65 % Ribonuclease: None detected % Deoxyribonuclease: None detected
    Biological Activity Comment
    31.4 units/mg
  • 应用备注

    Application Note: Proteinase K is specifically formulated for molecular cloning, nucleic acid research and protein/peptide structural analysis. One unit liberates one μmole of Folin positive amino acids, measured as tyrosine, at 37° C at  pH 7.5 using urea denatured hemoglobin as the substrate.

    说明

    Synonyms: Protease K, Endopeptidase K

    Background: Proteinase K is a broad-spectrum serine protease and is able to digest native keratin (hair), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of Proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液

    Buffer: None

    储存条件
    4 °C,-20 °C
    有效期
    6 months
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