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Quick Overview for TMB ELISA Peroxidase Substrate (ABIN925629)
应用范围
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Supplier Product No.
- tmbe-100
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Supplier
- Rockland
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原理
- TMB ELISA Peroxidase Substrate
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纯化方法
- pH: 4.0 +/- 0.2 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
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应用备注
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Application Note: TMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay
ELISA Dilution: 1X
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 x
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注意事项
- Protect from moisture and light.
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储存条件
- 4 °C
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储存方法
- Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required. Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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背景
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Synonyms: 3,3',5,5'-Tetramethylbenzidine, TMB ELISA substrate, TMBE Substrate, chromogenic ELISA substrate
Background: TMB (3,3',5,5'-Tetramethylbenzidine) is a colorimetric substrate for peroxidase (HRP)-based enzyme immunoassays, notably ELISAs, and is known for producing a blue color that changes to yellow upon stopping the reaction with sulfuric acid. The color change
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