Quick Overview for Blotto Immunoanalytical Grade (Non-Fat Dry Milk) (ABIN925606)
应用范围
Blocking Reagent (BR), Dot Blot (DB), Western Blotting (WB)
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Supplier Product No.
- b501-0500
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Supplier
- Rockland
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产品特性
- Supplier produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS (pH 7.5)BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS pH 7.210 X
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
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应用备注
- Use BLOTTO whenever procedures call for immunoanalytical grade non-fat dry milk. Alternative blocking agents such as BLOTTO A (solution of TBS, 5% non-fat dry milk and 0.05% Tween-20) and BLOTTO B (solution of TBS, 1% non-fat dry milk, 1% BSA and 0.05% Tween-20) are also available. BLOTTO A is a convenient general purpose blocking agent. BLOTTO B is recommended for blocking when phospho-specific antibodies are used. BLOTTO A and BLOTTO B provide Tween-20 in a separate vial in liquid form (p/n TW0025). Users should consider the addition of protease inhibitors and phosphatase inhibitors when required. Typically 50 mM NaF can be added to BLOTTO solutions to inhibit phosphatase activity.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Restore with deionized water (or equivalent)
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储存条件
- -20 °C
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