Quick Overview for BlockOut® Universal Blocking Buffer for Western Blotting (ABIN5706396)
应用范围
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Supplier Product No.
- mb-073
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Supplier
- Rockland
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原理
- BlockOut® Universal Blocking Buffer for Western Blotting
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品牌
- BlockOut®
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纯化方法
- BlockOut® Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.
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过滤
- Sterile filtered
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Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
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应用备注
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Application Note: BlockOut® allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with
Western Blot Dilution: User Defined
Other: Optimum performance is achieved using this product undiluted. However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 X
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- DO NOT FREEZE.
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储存条件
- 4 °C
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储存方法
- Store Blocking Buffer at 4° C prior to opening. DO NOT FREEZE. Expiration date is six (6) months from date of receipt.
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有效期
- 6 months
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背景
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Synonyms: Multiplex Blocking Buffer, Immunoblot Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer, colorimetric, chemiluminescent blocking buffer, WB block, BlockOut, B
Background: BlockOut® is an universal blocking buffer solution for Western Blotting. It is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting. Universal Blocking Buffer is recommended for blocking when phospho specific antibodi
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