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1X RIPA Buffer: Mix the 5X RIPA Buffer by inverting/vortexing the bottle a few minutes. Dilute the 5X RIPA Buffer to 1X with deionized water. Stir to homogeneity. Store at 4 °C for up to 6 months. Protease Inhibitor Cocktail: Immediately before use dilute the Protease Inhibitor Cocktail 1:100 with 1X RIPA Buffer. Stir to homogeneity. Do not store diluted solutions containing protease inhibitors.
I. Adherent Cells
II. Suspension Cells