Stop Solution for TMB Substrate
ELISA
Quick Overview for Stop Solution for TMB Substrate (ABIN2669207)
应用范围
ELISA
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产品特性
- Stop Solution for TMB Substrate is specifically formulated to terminate the HRP/TMB reaction and stabilizing the color development for ELISA applications. TMB is a chromogenic substrate used in ELISA. TMB can act as a proton donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as HRP. The resulting diimine causes the solution to take on a blue color, and this color change can be read at 650 nm. The reaction can be stopped by addition of stop reagent, and the color turns from blue to yellow with 2-3 fold increased absorbance values at 450 nm.
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组件
- Stop Solution for TMB Substrate 60 ml
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS (pH 7.5)BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS pH 7.210 X
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- RT
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