(4 references)
产品编号 ABIN1379874
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Perm/Wash Buffer I (ABIN1379874)
应用范围
Intracellular Flow Cytometry (ICFC)
-
-
品牌
- BD Phosflow™
-
产品特性
-
BD™ Phosflow Perm/Wash Buffer I is intended to be used for the intracellular staining of post-translationally modified signaling proteins.
BD™ Phosflow Perm/Wash Buffer I is used to permeabilize cells and to serve as an antibody diluent and cell wash buffer. It is optimized for use with the BD™ Phosflow brand of intracellular phosphorylated signaling protein-specific antibodies. BD™ Phosflow Perm/Wash Buffer I is provided as a 10X concentrated solution containing FBS and saponin. The presence of a small amount of precipitate may be observable and will not affect the performance of the buffer. Because saponin-mediated cell permeabilization is a reversible process, it is important to keep the cells in the presence of saponin during intracellular staining.
-
-
Blocking Buffer for Fluorescent Western Blotting
CA, ELISA, IF, IHC, IP, MA, WB 1 X
10X TTBS Buffer pH 7.5ELISA, WB 10 X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS Buffer pH 7.2ELISA, FACS, IF, WB 10 X
-
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Aqueous buffered solution containing saponin, fetal bovine serum and ≤ 0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store undiluted at 4°C. Do not freeze.
-
-
-
: "Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 55, Issue 2, pp. 61-70, (2003) (PubMed).
: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "Detection of intracellular cytokines by flow cytometry." in: Journal of immunological methods, Vol. 159, Issue 1-2, pp. 197-207, (1993) (PubMed).
: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).
-
: "Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 55, Issue 2, pp. 61-70, (2003) (PubMed).
-
You are here:
