(2 references)Quick Overview for Fixation Buffer (ABIN1379871)
应用范围
Intracellular Flow Cytometry (ICFC)
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品牌
- BD Cytofix™
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产品特性
- BD Cytofix™ Fixation Buffer is comprised of a neutral pH-buffered saline (i.e., Dulbecco's Phosphate-Buffered Saline) that contains 4% w/v paraformaldehyde. This fixation buffer is intended to preserve human and rodent lymphoid cells for the subsequent immunofluorescent staining of intracellular cytokines. BD Cytofix can also be used to preserve the light-scattering characteristics and fluorescence intensities of human and rodent hematopoietic cells that have been stained by immunofluorescence for subsequent flow cytometric analysis.
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Blocking Buffer for Fluorescent Western Blotting
BR, Lbl, WB 1 X
10X TTBS (pH 7.5)BCA, IA 10X
Fluoromount-G®FISH, ICC, IHC (fro), IHC (p), IHC (wm), PLA, TUNEL
10X PBS pH 7.210 X
RIPA Lysis BufferCLy, IP, RIA, WB 1 X
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C
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储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).
: "Paraformaldehyde fixation of hematopoietic cells for quantitative flow cytometry (FACS) analysis." in: Journal of immunological methods, Vol. 47, Issue 1, pp. 25-30, (1982) (PubMed).
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: "Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure." in: Immunological reviews, Vol. 119, pp. 65-93, (1991) (PubMed).
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