Catcher-Control
Quick Overview for Catcher-Control (ABIN6953228)
应用范围
Bead Matrix
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原理
- Catcher-Control is a control resin for all Catcher resins, which are based on 4 % crosslinked agarose beads.
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产品特性
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Catcher-Control is a control resin for all Catcher resins, which are based on 4 % cross-linked agarose beads. It was prepared using the same activation and quenching chemistry that was used for preparation of Catcher resins, but is not charged with any single-domain antibody.
Catcher-Control is thus the ideal resin for pre-depletion of extracts or as a side-by-side specificity control for any Catcher resins.
When using as a specificity control, Catcher-Control and the corresponding sdAb-charged Catcher Resin should be used under identical experimental conditions.
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应用备注
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Coating: Same chemistry as for Catcher resins, but without immobilized single-domain antibody.
Matrix: 4 % cross-linked agarose, bead size 50-150 μm
Buffer Compatibility:- Common buffer substances at pH 5 to 9
- 2 % Triton X-100, 1 % Tween-20, 1 % NP-40, 1 % CHAPS, 1 % Deoxycholate, 0.1 % SDS
- 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA
- 4 M urea
- 10 mM DTT, 10 mM 2-Mercaptoethanol
- RNAse A, DNAse I, Benzonase, protease inhibitors
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限制
- 仅限研究用
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缓冲液
- 50 % slurry in PBS containing 20 % Ethanol
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储存条件
- 4 °C
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储存方法
- Store at 4 °C, do not freeze
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有效期
- 12 months
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