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Catcher-Control

ChIP, IP, Co-IP Agarose beads
产品编号 ABIN6953228
发货至: 中国

Quick Overview for Catcher-Control (ABIN6953228)

应用范围

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Protein Complex Immunoprecipitation (Co-IP)

Bead Matrix

Agarose beads
  • 原理

    Catcher-Control is a control resin for all Catcher resins, which are based on 4 % crosslinked agarose beads.

    产品特性

    Catcher-Control is a control resin for all Catcher resins, which are based on 4 % cross-linked agarose beads. It was prepared using the same activation and quenching chemistry that was used for preparation of Catcher resins, but is not charged with any single-domain antibody.
    Catcher-Control is thus the ideal resin for pre-depletion of extracts or as a side-by-side specificity control for any Catcher resins.
    When using as a specificity control, Catcher-Control and the corresponding sdAb-charged Catcher Resin should be used under identical experimental conditions.
  • 应用备注

    Coating: Same chemistry as for Catcher resins, but without immobilized single-domain antibody.
    Matrix: 4 % cross-linked agarose, bead size 50-150 μm
    Buffer Compatibility:
    • Common buffer substances at pH 5 to 9
    • 2 % Triton X-100, 1 % Tween-20, 1 % NP-40, 1 % CHAPS, 1 % Deoxycholate, 0.1 % SDS
    • 4 M NaCl, 2 M KCl, 1 M MgCl2, 100 mM EDTA
    • 4 M urea
    • 10 mM DTT, 10 mM 2-Mercaptoethanol
    • RNAse A, DNAse I, Benzonase, protease inhibitors

    限制

    仅限研究用
  • 缓冲液

    50 % slurry in PBS containing 20 % Ethanol

    储存条件

    4 °C

    储存方法

    Store at 4 °C, do not freeze

    有效期

    12 months
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