Protein A/G Magnetic Beads
IP, Purif, AC
Protein A,Protein G
Magnetic particles
40 µm
(2 references)Quick Overview for Protein A/G Magnetic Beads (ABIN400577)
抗原
Protein A/G
应用范围
Immunoprecipitation (IP), Purification (Purif), Affinity Chromatography (AC)
Bead Ligand
Protein A,Protein G
Bead Matrix
Magnetic particles
Bead Size
40 µm
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原理
- Protein A/G MagBeads are designed for small-scale antibody purification and immunoprecipitation of proteins, protein complexes or other antigens.
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品牌
- MagBeads
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产品特性
- Protein A/G MagBeads are superparamagnetic beads of average 40 μm in diameter, covalently coated with recombinant Protein A/G. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein A/G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 0.5 ml settled Beads (2 ml 25% slurry)
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储存条件
- 4 °C
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储存方法
- Store at 4°C, do NOT freeze.
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有效期
- 12 months
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: "Unassembled CD147 is an endogenous endoplasmic reticulum-associated degradation substrate." in: Molecular biology of the cell, Vol. 23, Issue 24, pp. 4668-78, (2012) (PubMed).
: "Human cytomegalovirus infection causes premature and abnormal differentiation of human neural progenitor cells." in: Journal of virology, Vol. 84, Issue 7, pp. 3528-41, (2010) (PubMed).
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: "Unassembled CD147 is an endogenous endoplasmic reticulum-associated degradation substrate." in: Molecular biology of the cell, Vol. 23, Issue 24, pp. 4668-78, (2012) (PubMed).
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- Protein A/G
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背景
- Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6×His-tag was attached to its N-terminal to facilitate the purification. The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more universal tool to bind and purify immunoglobulins.
抗原
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