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北京 101111
Quick Overview for High Affinity Ni-Charged Resin (ABIN1536567)
应用范围
Bead Ligand
Bead Matrix
Bead Size
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特异性
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High binding capacity: The kit can handle over 20 mg 6xHis-tagged protein/mL (CV).
Simple purification procedure under both native and denaturing conditions.
Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
Resilient: If properly regenerated, the resin can be reused multiple times. -
产品特性
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High Affinity Ni-Charged Resin is an 4 % cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins. High Affinity Ni-Charged Resin is available in 10, 25 and 500 mL lab packs.
Total Volume: 10 mL settled resin (20 mL 50 % slurry) 25 mL settled resin (50 mL 50 % slurry) 500 mL settled resin (1000 mL 50 % slurry)
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Magnetic Concanavalin A Beads (Agarose)
CUT&RUN, CUT&Tag, Sep Concanavalin A Magnetic Agarose beads 30 μm
Ni-NTA MagBeadsPull-Down, Purif, Sep Ni-NTA 25 µm
Ni-NTA AgarosePurif, Sep Ni-NTA 40 µm
Rho1D4 AgarosePurif, Sep Rho1D4 40 µm
GFP-CatcherCo-IP, IP, Purif, ChIP, RIP Antibody Agarose beads 90 μm
BFP-CatcherCo-IP, IP, Purif, ChIP, RIP Antibody Agarose beads 90 μm
INDIGO Ni-AgarosePurif Ni-INDIGO Agarose beads 100 μm
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说明
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For ambient pressure use only.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C
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储存方法
- Store the product between 2°C and 8°C.
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: "Characterization of the insertase BamA in three different membrane mimetics by solution NMR spectroscopy." in: Journal of biomolecular NMR, Vol. 61, Issue 3-4, pp. 333-45, (2015) (PubMed).
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