Streptavidin Resin
Quick Overview for Streptavidin Resin (ABIN1536312)
抗原
应用范围
Bead Ligand
Bead Matrix
Bead Size
-
-
原理
- Streptavidin Resin is an affinity chromatography medium designed for easy, one-step purification of biotinylated peptides, antibodies, lectins, etc from samples.
-
特异性
-
Resin Volume 5 ml settled resin (10 ml 50% slurry)
Ligand Streptavidin, affinity purified with IminoBiotin Resin (L00272)
Number of biotin binding sites per ligand 4
M.W. of ligand Approximately 54 kDa
PI of ligand 6.52
Degree of substitution Approximately 2 mg streptavidin/ml settled resin
Total binding capacity > 120 nmol of D-Biotin/ml settled resin
Matrix spherical agarose, 4% cross-linked
Average particle size 90 μm (45 - 165 μm)
Storage solution 1X PBS containing 20% ethanol
Storage conditions 2 - 8°C
Shelf life 12 months when stored unopened -
产品特性
- The purified recombinant streptavidin is covalently coupling to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for biotinylated molecules. The total binding capacity of Streptavidin Resin is more than 120 nmol of D-Biotin/ml settled resin.
-
-
MagSi-STA 1.0 beads
Sep, IA Streptavidin Magnetic Silica particles 1 µm
MagSi-STA 1.0 TL beadsSep, IA Streptavidin Magnetic Silica particles 1 µm
MagSi-STA 3.0 TL beadsSep, IA Streptavidin Magnetic Silica particles 3 µm
MagSi-STA 1.0 L beadsSep, IA Streptavidin Magnetic Silica particles 1 µm
MagSi-STA 3.0 L beadsSep, IA, Purif Streptavidin Magnetic Silica particles Bead size: 3 µm
MagSi-STA 1.0 TS beadsSep, IA Streptavidin Magnetic Silica particles 1 µm
MagSi-STA 600 beadsSep, IA Streptavidin Magnetic Silica particles 600 nm
MagSi-STA 600 BI beadsSep, IA Streptavidin Magnetic Silica particles 600 nm
MagSi-STA 3.0 L beadsSep, IA, Purif Streptavidin Magnetic Silica particles Bead size: 3 µm
MagSi-STA 1.0 TS beadsSep, IA Streptavidin Magnetic Silica particles 1 µm
-
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- 1X PBS containing 20% ethanol
-
注意事项
- High temperature heating is not recommended. The agarose melts above 65°C.
-
储存条件
- 4 °C
-
有效期
- 12 months
-
-
- Streptavidin
-
背景
- Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Streptavidin binds 4 moles of biotin per mole of protein with an extremely high affinity. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point of 6.5 (vs 10 for avidin) close to where most useful biological interactions occur. As a result, streptavidin frequently exhibits much lower non-specific binding than avidin does.
-
分子量
- ~ 54 kDa
抗原
-
