(19 references)
产品编号 ABIN1536254
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Protein G Resin (ABIN1536254)
抗原
Protein G
应用范围
Purification (Purif), Affinity Chromatography (AC)
Bead Ligand
Protein G
Bead Matrix
Agarose beads
Bead Size
90 µm
-
-
适用
- Streptococcus
-
原理
- Protein G Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media.
-
产品特性
-
The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin is greater than 20 mg sheep IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc.
Ligand: Recombinant Streptococcal Protein G lacking the albumin-binding produced in E. coli
- Number of IgG binding sites per ligand: 3
- MW of ligand: Approximately 22 kDa
- PI of ligand: 4.69
- Degree of substitution: Approximately 2 mg protein G/ml settled resin
- Static binding capacity: >20 mg sheep IgG/ml settled resin
- Matrix spherical: agarose, 4% cross-linked
- Average particle size: 90 μm (45-165 μm)
-
-
MagSi-protein G 1.0 beads
Purif, IP, Sep Protein G Magnetic Silica particles 1 µm
MagSi-protein G 600 beadsPurif, IP, Sep Protein G Magnetic Silica particles 600 nm
Protein G SepharosePurif, IP Protein G Sepharose beads 90 µm
MagSi-protein G 1.0 beadsPurif, IP, Sep Protein G Magnetic Silica particles Bead size: 1.0 µm
MagSi-protein G 600 beadsPurif, IP, Sep Protein G Magnetic Silica particles Bead size: 600 nm
-
-
说明
-
High temperature heating is not recommended. The agarose melts above 65°C.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- 1X PBS containing 20% ethanol
-
注意事项
- High temperature heating is not recommended. The agarose melts above 65°C.
-
储存条件
- 4 °C
-
储存方法
- Store regenerated Protein G Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
-
有效期
- 18 months
-
-
-
: "Calcitriol exerts anti-inflammatory effects in keratinocytes treated with autoantibodies from a patient with bullous pemphigoid." in: Journal of the European Academy of Dermatology and Venereology : JEADV, (2015) (PubMed).
: "Development of monoclonal antibodies against Plasmodium falciparum thioredoxin peroxidase 1 and its possible application for malaria diagnosis." in: Experimental parasitology, Vol. 154, pp. 62-6, (2015) (PubMed).
: "Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa." in: Fungal genetics and biology : FG & B, Vol. 80, pp. 10-8, (2015) (PubMed).
: "The combined use of photoaffinity labeling and surface plasmon resonance-based technology identifies multiple salicylic acid-binding proteins." in: The Plant journal : for cell and molecular biology, Vol. 72, Issue 6, pp. 1027-38, (2014) (PubMed).
: "A CD40/CD40L feedback loop drives the breakdown of CD8(+) T-cell tolerance following depletion of suppressive CD4(+) T cells." in: European journal of immunology, Vol. 44, Issue 4, pp. 1099-107, (2014) (PubMed).
: "Divergent outcomes following transcytosis of IgG targeting intracellular and extracellular chlamydial antigens." in: Immunology and cell biology, Vol. 92, Issue 5, pp. 417-26, (2014) (PubMed).
: "M3 muscarinic receptor interaction with phospholipase C ?3 determines its signaling efficiency." in: The Journal of biological chemistry, Vol. 289, Issue 16, pp. 11206-18, (2014) (PubMed).
: "A novel histone deacetylase complex in the control of transcription and genome stability." in: Molecular and cellular biology, Vol. 34, Issue 18, pp. 3500-14, (2014) (PubMed).
: "Generation and characterization of polyclonal antibody against part of immunoglobulin constant heavy ? chain of goose." in: Monoclonal antibodies in immunodiagnosis and immunotherapy, Vol. 33, Issue 4, pp. 287-90, (2014) (PubMed).
: "Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development." in: FEBS letters, Vol. 588, Issue 20, pp. 3726-31, (2014) (PubMed).
: "Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish." in: Developmental biology, Vol. 395, Issue 2, pp. 268-86, (2014) (PubMed).
: "dCREB2-mediated enhancement of memory formation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 17, pp. 7475-87, (2013) (PubMed).
: "Antibodies mediate formation of neutrophil extracellular traps in the middle ear and facilitate secondary pneumococcal otitis media." in: Infection and immunity, Vol. 82, Issue 1, pp. 364-70, (2013) (PubMed).
: "Wnt3a-stimulated LRP6 phosphorylation is dependent upon arginine methylation of G3BP2." in: Journal of cell science, Vol. 125, Issue Pt 10, pp. 2446-56, (2012) (PubMed).
: "Release of dendritic cells from cognate CD4+ T-cell recognition results in impaired peripheral tolerance and fatal cytotoxic T-cell mediated autoimmunity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 23, pp. 9059-64, (2012) (PubMed).
: "The posttranslational modification of tubulin undergoes a switch from detyrosination to acetylation as epithelial cells become polarized." in: Molecular biology of the cell, Vol. 22, Issue 7, pp. 1045-57, (2011) (PubMed).
: "Heat-shock protein gp96/grp94 is an essential chaperone for the platelet glycoprotein Ib-IX-V complex." in: Blood, Vol. 117, Issue 26, pp. 7136-44, (2011) (PubMed).
: "Solid phase single-molecule counting of antibody binding to supported protein layers surface with low nonspecific adsorption." in: Talanta, Vol. 82, Issue 3, pp. 1003-9, (2010) (PubMed).
: "CTA4 transcription factor mediates induction of nitrosative stress response in Candida albicans." in: Eukaryotic cell, Vol. 7, Issue 2, pp. 268-78, (2008) (PubMed).
-
: "Calcitriol exerts anti-inflammatory effects in keratinocytes treated with autoantibodies from a patient with bullous pemphigoid." in: Journal of the European Academy of Dermatology and Venereology : JEADV, (2015) (PubMed).
-
- Protein G
-
背景
-
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
Broad IgG binding spectrum
Binding specificity complements of protein A
Agarose media
No specific albumin binding
Optimized homogeneous recombinant ligand
High capacity
抗原
-
You are here:
