Aubergine 抗体 (Internal Region)
This anti-Aubergine antibody is a 兔 多克隆 antibody detecting Aubergine in ELISA 和 WB. Suitable for Drosophila melanogaster.
Quick Overview for Aubergine 抗体 (Internal Region) (ABIN129699)
抗原
Aubergine (aub)
适用
Drosophila melanogaster
宿主
兔
克隆类型
多克隆
应用范围
ELISA, Western Blotting (WB)
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-947
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Supplier
- Rockland
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原理
- Aubergine/Sting Antibody
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交叉反应 （详细）
- Anti-Aubergine affinity-purified antibody is directed against Drosophila Aubergine protein.
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产品特性
- Synonyms: rabbit anti-Aubergine/Sting Antibody, rabbit anti-Aubergine Antibody, rabbit anti-Sting Antibody, Aubergine isoform A
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Drosophila melanogaster Aubergine/Sting protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Application Note: Aubergine affinity-purified antibody has been tested for use in ELISA. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 98 kDa in size corresponding to Aubergine protein is expected in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000 - 1:10,000
ELISA Dilution: 1:20,000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Aubergine (aub)
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别名
- aub
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物质类
- Species
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背景
- Background: Aubergine (Sting) is a Drosophila protein involved in germ-line development and RNA interference. Drosophila melanogaster has a robust and efficient innate immune system, which reacts to infections ranging from bacteria to fungi and, as discovered recently, viruses as well. The known Drosophila immune responses rely on humoral and cellular activities, similar to those found in the innate immune system of other animals. Recently, RNAi or 'RNA silencing' has arisen as a possible means by which Drosophila can react to specific pathogens, transposons and retroviral elements, in a fashion similar to that of a traditional mammalian adaptive immune system instead of in a more generalized and genome encoded innate immune-based response. RNAi is a highly conserved regulation and defense mechanism, which suppresses gene expression via targeted RNA degradation directed by either exogenous dsRNA (cleaved into siRNAs) or endogenous miRNAs. In plants, RNAi has been found to act as an antiviral immune response system. In Drosophila multiple core RNAi pathway genes have been reported, including piwi, vasa intronic gene (vig), aubergine (aub), armitage (armi), Rm62, r2d2 and Argonaute2 (AGO2).
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基因ID
- 34524, 17136494
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UniProt
- O76922
抗原
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