人 Glycosylation Array 493
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- 适用
- 人
- 检测方法
- Fluorometric
- 应用范围
- Antibody Array (AA)
- 原理
- Human Glycosylation Antibody Array 493. For the simultaneous detection of the glycosylation protein profile of 493 human proteins in serum, plasma, cell culture supernatants, cell/tissue lysates or other body fluids.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum, Cell Lysate, Tissue Lysate
- Analytical Method
- Semi-Quantitative
- 特异性
- 11b-HSD1, 2B4, 4-1BB, A1BG, A2M, ABL1, ACE, ACE-2, ACK1, ACPP, ACTH, ADAM-9, ADAMTS-1, ADAMTS-10, ADAMTS-13, ADAMTS-15, ADAMTS-17, ADAMTS-18, ADAMTS-19, ADAMTS-4 , ADAMTS-5, ADAMTS-L2, Adipsin, Afamin, AFP, ALBUMIN, Aldolase A, Aldolase B, Aldolase C, ALK, Alpha 1 AG, Alpha 1 Microglobulin, Alpha Lactalbumin, ALPP, AMICA, AMPKa1, Amylin, ANGPTL3, ANGPTL4, Annexin A7, APC, APCS, Apelin, Apex1, APN, ApoA1, ApoA2, ApoA4, ApoB, ApoB100, ApoC1, ApoC2, ApoC3, ApoD, ApoE, ApoE3, ApoH, ApoM, APP, ASPH, Attractin, B3GNT1, BAF57, BAFF, BAI-1, BCAM, Beta 2M, Beta Defensin 4, Beta IG-H3, Biglycan, BLAME, BMP-9, BMX, BNIP2, BNP, Btk, C2, C3a, C5/C5a, C7, C8B, C9, CA125, CA15-3, CA19-9, CA9, Cadherin-13, Calbindin, Calbindin D, Calcitonin, Calreticulin, Calsyntenin-1, CART, Caspase-3, Caspase-8, Cathepsin B, Cathepsin D, Cathepsin L, Cathepsin S, CBP, CCK, CD200, CD23, CD24, CD36, CD38, CD44, CD45, CD46, CD47, CD55, CD59, CD61, CD71, CD74, CD79 alpha, CD90, CD97, CEA, CEACAM-1, Ceruloplasmin, CFHR2, Chemerin, CHI3L1, Chromogranin A, Chymase, cIAP-2, Ck beta 8-1, CK-MB, Claudin-3, Claudin-4, CLEC3B, Clusterin, CNDP1, COCO, Complement factor H, Contactin-1, Contactin-2, Corticosteroid-binding globulin, COX-2, C-peptide, CPN2, Creatinine, CRP, CRTAM, CSH1, cTnT / Troponin T, CutA, Cyclin D1, Cystatin A, Cystatin B, Cystatin C, Cytochrome C, Cytokeratin 8, Cytokeratin18, Cytokeratin19, DBI, DCBLD2, D-Dimer, DEFA1/3, Defensin, Desmin, DLL1, DLL4, DMP-1, DPPIV, E-Cadherin, Endorphin Beta, Endothelin Receptor A, Enolase 2, ENPP2, EpCAM, EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, EphB1, EphB2, EphB3, EphB4, EphB6, ERRa, Erythropoietin R, ESAM, EV15L, EXTL2, FABP1, FABP2, FABP3, FABP4, Factor XIII A, Factor XIII B, FAK, FAP, Fc RIIB/C, Fen 1, FER, Ferritin, Fetuin A, Fetuin B, FGFR1, FGFR1 alpha, FGFR2, Fibrinogen, Fibrinopeptide A, Fibronectin, Ficolin-3, FIH, FOLR1, FOXN3, FoxO1, FoxP3, FRK, FSH, Furin, Fyn, GADD45A, Galanin, Galectin-1, Galectin-3BP, Galectin-7, gamma-Thrombin, Gas1, Gastrin, GATA-3, GATA-4, Gelsolin, Ghrelin, GLP-1, GMNN, GPBB, GPI, GPR-39, GPX1, GPX3, GRP, GRP75, GRP78, GSR, GST, HADHA, HAI-1, HAI-2, Haptoglobin, hCG alpha, hCGb, Hck, HE4, Hemopexin, Hepcidin, HOXA10, HSP10, HSP20, HSP27, HSP32, HSP40, HSP60, HSP70, HSP90, HSPA8, HTRA2, IBSP, IGF2BP1, IGFBP-5, IL-23p19, IL-33, IL-34, IL36RN, INSL3, INSRR, Integrin alpha V, Itk, ITM2B, Kallikrein 10, Kallikrein 11, Kallikrein 14, Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, KCC3, KCTD10, KIF3B, KLF4, LAG-3, Layilin, LDL R, Legumain, LH, LIMPII, LIN41, Livin, LOX-1, LPS, LRG1, LTF, LTK, Lumican, Lyn, LYRIC, LYVE-1, LZTS1, Mammaglobin A, Marapsin, MATK, MBL, MBL-2, Mer, Mesothelin, MICB, Midkine, MINA, MSHa, MTUS1, Myoglobin, NAIP, Nanog, NELL2, NEP, Nesfatin, Nestin, NET1, Netrin G2, Netrin-4, Neurokinin-A, Neuropeptide Y, NF1, NM23-H1/H2, Notch-1, NPTX1, NPTXR, NR3C3, Ntn1, OCT3/4, Omentin, Osteocalcin, Osteopontin, OX40, p21, p27, p53, PAI-1, PAK7, Pancreastatin, Pancreatic Polypeptide, Pappalysin-1, PARK7, P-Cadherin, PCAF, PD-1, PDX-1, PEDF , PEPSINOGEN I, PEPSINOGEN II, PGRP-S, PI 16, PI 3Kinase p85 beta, PIM2, PKM2, Plasminogen, Podocalyxin, POMC, PON1, PON2, PPARg2, PPP2R5C, Presenilin 1, Presenilin 2, Pro-BDNF, Procalcitonin, Pro-Cathepsin B, Progesterone, pro-Glucagon, Prohibitin, Pro-MMP-13, Pro-MMP-7, Pro-MMP-9, ProSAAS, Prostasin, Protein p65, PSA-Free, PSA-total, PSP, PTH, PTHLP, PTN, PTPRD, PYK2, PYY, Ras, RBP4, RECK, RELM alpha, Resistin, RET, RIP1, ROCK1, ROCK2, ROR1, ROR2, ROS, RYK, S100A4, S100A6, S100A8, S-100b, SART1, SART3, SCG3, Selenoprotein P, SEMA3A, Serotonin, Serpin A1, Serpin A12, Serpin A3, Serpin A4, Serpin A5, Serpin A8, Serpin A9, Serpin B5, Serpin D1, Serpin I1, SERTAD2, SHBG, SMAC, SNCG, Somatotropin, SOST, SOX17, SOX2, SPARCL1, SPINK1, SRMS, SSEA-1, SSEA-4, SSTR2, SSTR5, Survivin, SYK, Syndecan-1, Syndecan-3, TACE, TAF4, Tec, TFF1, TFF3, Thrombin, Thrombomodulin, Thymidine Kinase-1, Thyroglobulin, TIM-1, TNK1, TOPORS, TPA, TPM1, TRA-1-60, TRA-1-81, Transferrin, Trappin-2, TRKB, Troponin C, Troponin I, TRPC1, TRPC6, TRPM7, Trypsin 1, TSH, TSLP, TXK, Tyk2, TYRO10, Uromodulin, Vasopressin, VDUP-1, VEGF R1, VGF, VIP Receptor 2, Visfatin, Vitamin D Receptor, Vitamin D-BP, Vitamin K-dependent protein S, Vitronectin, VWF, Wilms Tumor 1, XIAP, ZAG, ZAP70
- 产品特性
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- High density screening
- High sensitivity and specificity
- Low sample volume (20-100 μL per array)
- Large dynamic range of detection
- Compatible with most sample types
- Accurate and reproducible
- 组件
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Human Glycosylation Array Glass Slide/s
Dilution Buffer
20X Wash Buffer I
20X Wash Buffer II
Cy3 equivalent dye-conjugated Streptavidin
Biotin-labeled Lectin Mixture
Adhesive device sealer
Manual - 试剂未包括
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Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Distilled or de-ionized water
1 mL microcentrifuge tubes
Gene microarray scanner or similar laser fluorescence scanner
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Human Cytokine Array C5
适用: 人 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
Human Cytokine Array C3适用: 人 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
Human Cytokine Array C1适用: 人 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
Human Angiogenesis Array C1适用: 人 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
Human Cytokine Array C6适用: 人 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
Human Cytokine Array C7适用: 人 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
Mouse Cytokine Array C3适用: 小鼠 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
Human Inflammation Array C3适用: 人 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
Mouse Cytokine Array C1适用: 小鼠 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
Human Apoptosis Array C1适用: 人 AA Chemiluminescent Semi-Quantitative Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate Membrane
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- 说明
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Glycome Arrays allow researchers to efficiently obtain glycosylation profiles in their samples. Capture antibodies are printed onto glass slides and the glycans on these capture antibodies are removed. The glass slide arrays come pre-blocked and are ready to be incubated with samples. After incubation with samples and washing to remove unbound proteins, five unique biotin-labeled lectins are incubated with the array to allow binding to the glycans from captured target proteins. Streptavidin-conjugated fluorescent dye (Cy3 equivalent) is then added to the array. Finally, the glass slide is dried and laser fluorescence scanning is used to visualize the signals. These signals are then compared to the array map to identify glycosylated proteins present in the samples.
- 样本量
- 50 μL
- 板类型
- Glass Slide
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- Upon receipt, all components of the Human Glycosylation Array 493 kit should be stored at -20°C. Once thawed, the glass slide and Cy3 equivalent dye-conjugated Streptavidin should be kept at -20°C and all other components may be stored at 4°C. The entire kit should be used within 6 months of purchase.
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