AOX1/AOX2 抗体 (C-Term)
This anti-AOX1/AOX2 antibody is a 兔 多克隆 antibody detecting AOX1/AOX2 in WB. Suitable for Arabidopsis thaliana, Lupinus luteus, Moss, Nicotiana tabacum, Pisum sativum 和 Potato. This Primary Antibody has been cited in 7+ publications.
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(7 references)Quick Overview for AOX1/AOX2 抗体 (C-Term) (ABIN3197483)
抗原
AOX1/AOX2
(Alternative Oxidase 1/2 (AOX1/AOX2))
适用
Arabidopsis thaliana, Lupinus luteus, Moss, Nicotiana tabacum, Pisum sativum, Potato
宿主
兔
克隆类型
多克隆
应用范围
Western Blotting (WB)
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抗原表位
- C-Term
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交叉反应 （详细）
- No cross-reactivity with: Chlamydomonas reinhardtii
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产品特性
- Expected / apparent Molecular Weight of the Antigene: 36-40 / 36-40 for Arabidopsis thaliana
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纯化方法
- serum
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免疫原
- KLH-conjugated synthetic peptide derived from fully conserved C-terminal consensus motif from plant AOX isoforms 1 and 2 including Arabidopsis thaliana AOX1A (At3g22370) and AOX2 (At3g64210), Solanum lycopersicum Q7XBG9, Oryza sativa Q7XT33,
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应用备注
- Recommended Dilution: 1 : 1000 on 10-20 µg of mitochondrial protein/lane with standard ECL detection(WB).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 200 µL of sterile water
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注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
储存条件
- -20 °C
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: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).
: "Low temperature storage affects the ascorbic acid metabolism of cherry tomato fruits." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 84, pp. 149-57, (2014) (PubMed).
: "Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria." in: Mitochondrion, Vol. 19 Pt B, pp. 222-30, (2014) (PubMed).
: "A lack of mitochondrial alternative oxidase compromises capacity to recover from severe drought stress." in: Physiologia plantarum, (2013) (PubMed).
: "Respiratory flexibility and efficiency are affected by simulated global change in Arctic plants." in: The New phytologist, Vol. 197, Issue 4, pp. 1161-72, (2013) (PubMed).
: "Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana." in: Proteomics, Vol. 11, Issue 9, pp. 1780-8, (2011) (PubMed).
: "Nitric oxide increases mitochondrial respiration in a cGMP-dependent manner in the callus from Arabidopsis thaliana." in: Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society, (2010) (PubMed).
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: "Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response." in: BMC plant biology, Vol. 14, pp. 144, (2014) (PubMed).
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- AOX1/AOX2 (Alternative Oxidase 1/2 (AOX1/AOX2))
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别名
- Plant alternative oxidase 1 and 2 (AOX1/2)
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背景
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AGI Code: At3g22370
Alternative oxidases (AOX) are quinol oxidases located in the inner mitochondrial membrane of plants. They function as terminal oxidases in the alternate electron transport pathway, oxidizing ubiquinone to reduce oxygen to water. -
分子量
- expected: 36-40 kDa, apparent: 36-40 for Arabidopsis thaliana
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UniProt
- Q7XBG9, Q7XT33, O22049, Q39219
抗原
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