GPD1 抗体
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- 抗原 See all GPD1 抗体
- GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))
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适用
- 兔
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GPD1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-101-210-0100
- Supplier
- Rockland
- 原理
- Glycerol-3-Phosphate Dehydrogenase Antibody
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glycerol-3-Phosphate Dehydrogenase [Rabbit Muscle].
- 产品特性
- Synonyms: goat anti-Glycerol-3-Phosphate Dehydrogenase Antibody, FLJ26652 antibody, G3PD antibody, Gdc-1 antibody, Glycerphosphate dehydrogenase antibody, GPD-C antibody, Gpd1 protein antibody
- 纯化方法
- Anti-GLYCEROL-3-PHOSPHATE DEHYDROGENASE (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Glycerol-3-Phosphate Dehydrogenase [Rabbit Muscle]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product GPD1 Primary Antibody
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- 应用备注
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Application Note: Anti-GLYCEROL-3-PHOSPHATE DEHYDROGENASE (GOAT) Antibody has been assayed against 1.0 μg of Glycerol-3-Phosphate Dehydrogenase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by researcher.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- GPD1 (Glycerol-3-Phosphate Dehydrogenase 1 (Soluble) (GPD1))
- 别名
- GPD1 (GPD1 产品)
- 背景
- Background: Glycerol-3-phosphate dehydrogenase (GPDH) is an enzyme that catalyzes the reversible redox conversion of dihydroxyacetone phosphate (aka glycerone phosphate, outdated) to sn-glycerol 3-phosphate. Glycerol-3-phosphate dehydrogenase serves as a major link between carbohydrate metabolism and lipid metabolism. It is also a major contributor of electrons to the electron transport chain in the mitochondria. Older terms for glycerol-3-phosphate dehydrogenase include alpha glycerol-3-phosphate dehydrogenase (alphaGPDH) and glycerolphosphate dehydrogenase (GPDH). However, glycerol-3-phosphate dehydrogenase is not the same as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) whose substrate is an aldehyde not an alcohol. Anti-Glycerol-3-Phosphate Dehydrogenase Antibody is ideal for investigators involved in glucose energy metabolism research.
- 基因ID
- 100339469, 3043365
- UniProt
- P08507
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