Glycerol Kinase 抗体 (Biotin)
Quick Overview for Glycerol Kinase 抗体 (Biotin) (ABIN99893)
抗原
See all Glycerol Kinase (GK) 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-106-211-0100
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Supplier
- Rockland
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原理
- Glycerol Kinase Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glycerol Kinase [Cellulomonas species].
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产品特性
- Synonyms: goat anti-Glycerol Kinase Antibody biotin Conjugation, biotin Conjugated goat anti-Glycerol Kinase Antibody, ATP glycerol 3 phosphotransferase antibody, GK antibody, GK antibody, GK1 antibody, GKD antibody, Glycerokinase antibody, Glycerol kinase antibody, Glycerol kinase deficiency antibody
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纯化方法
- Anti-Glycerol Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glycerol Kinase [Cellulomonas species]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Glycerol Kinase (GK) (AA 1-524) antibody
GK 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Glycerol Kinase (GK) antibodyGK 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 2-94) antibodyGK 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 1-524) antibodyGK 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) antibodyGK 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 1-180) antibodyGK 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 21-120) antibodyGK 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 2-502) antibodyGK 适用: E. coli ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Glycerol Kinase (GK) (AA 2-502) antibody (Biotin)GK 适用: E. coli ELISA, WB 宿主: 兔 Polyclonal Biotin
anti-Glycerol Kinase (GK) (AA 461-510) antibodyGK 适用: 人, 小鼠, 大鼠 ELISA, WB, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Glycerol Kinase has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Glycerol Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Glycerol Kinase (GK)
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别名
- glpK
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背景
- Background: Anti-Glycerol Kinase antibody recognizes the phosphotransferase enzyme glycerol kinase. Glycerol kinase catalyzes the formation of glycerol phosphate by transferring a phosphate from ATP to glycerol. Dihydroxyacetone and L-glyceraldehyde can also act as acceptors in place of glycerol. Anti-Glycerol Kinase is ideal for investigators interested in Cancer, Metabolism, and Kinase/Phosphatase enzymology.
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基因ID
- 6960048
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UniProt
- D0VZG4
抗原
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