GST 抗体 (HRP)
(2 references)
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- 抗原 See all GST 抗体
- GST (Glutathione S Transferase (GST))
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适用
- Schistosoma japonicum
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This GST antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 600-103-200
- Supplier
- Rockland
- 原理
- GST Antibody HRP Conjugated
- 交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
- 产品特性
- Synonyms: goat anti-GST antibody HRP conjugation, peroxidase conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody
- 纯化方法
- Anti-GST HRP Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
- 免疫原
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Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product GST Primary Antibody
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anti-Glutathione S Transferase (GST) antibody
GST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibody (Biotin)GST 适用: 化学剂 WB, ELISA 宿主: 小鼠 Monoclonal vpg66 Biotin
anti-Glutathione S Transferase (GST) antibodyGST 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1-00E-05 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP 宿主: 小鼠 Monoclonal 2F10B9 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) (AA 1-218) antibodyGST 适用: Various Species WB, IP, DB 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) (AA 1-218) antibodyGST 适用: Various Species WB, IP, DB 宿主: 小鼠 Monoclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibody (Alkaline Phosphatase (AP))GST 适用: Schistosoma japonicum WB, ELISA 宿主: 小鼠 Monoclonal 3E2 Alkaline Phosphatase (AP)
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- 应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,000
Application Note: Anti-GST peroxidase conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using peroxidase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.
Western Blot Dilution: 1:2,000 - 1:5,000
ELISA Dilution: 1:20,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! - 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store anti-GST at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "NHDL, a recombinant VL/VH hybrid antibody control for IgG2/4 antibodies." in: mAbs, Vol. 12, Issue 1, pp. 1686319, (2020) (PubMed).
: "IgG Fc domains that bind C1q but not effector Fcγ receptors delineate the importance of complement-mediated effector functions. ..." in: Nature immunology, Vol. 18, Issue 8, pp. 889-898, (2017) (PubMed).
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: "NHDL, a recombinant VL/VH hybrid antibody control for IgG2/4 antibodies." in: mAbs, Vol. 12, Issue 1, pp. 1686319, (2020) (PubMed).
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- 抗原
- GST (Glutathione S Transferase (GST))
- 别名
- GST (GST 产品)
- 背景
- Background: GST Antibody HRP Conjugated is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag. Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
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