GST 抗体 (Biotin)
(1 reference)
Quick Overview for GST 抗体 (Biotin) (ABIN99864)
抗原
See all GST 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-106-200
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Supplier
- Rockland
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原理
- GST Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
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产品特性
- Synonyms: goat anti-GST antibody biotin conjugation, biotin conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody
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纯化方法
- Anti-GST Antibody, Biotin Conjugated, was prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Glutathione S Transferase (GST) antibody
GST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibody (Biotin)GST 适用: 化学剂 WB, ELISA 宿主: 小鼠 Monoclonal vpg66 Biotin
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP 宿主: 小鼠 Monoclonal 2F10B9 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Schistosoma japonicum WB, ELISA, IP, DB 宿主: 小鼠 Monoclonal GST-B6 unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: Nicotiana tabacum WB 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB 宿主: 兔 Polyclonal unconjugated
anti-Glutathione S Transferase (GST) antibodyGST 适用: 标记 WB, ELISA, IP, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 6G9C6 unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-GST Antibody, Biotin Conjugated has been tested by ELISA and is suitable for immunoblotting, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "The Identification of Naturally Occurring Neoruscogenin as a Bioavailable, Potent, and High-Affinity Agonist of the Nuclear Receptor RORα (NR1F1)." in: Journal of biomolecular screening, (2013) (PubMed).
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: "The Identification of Naturally Occurring Neoruscogenin as a Bioavailable, Potent, and High-Affinity Agonist of the Nuclear Receptor RORα (NR1F1)." in: Journal of biomolecular screening, (2013) (PubMed).
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- GST (Glutathione S Transferase (GST))
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别名
- GST
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背景
- Background: Biotin conjugated anti-GST antibody is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.
抗原
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