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GST 抗体 (Alkaline Phosphatase (AP))

GST 适用: Schistosoma japonicum WB, ELISA, IHC 宿主: 山羊 Polyclonal Alkaline Phosphatase (AP)
Rockland
产品编号 ABIN99863
Supplier Product No.: 600-105-200
发货至: 中国
  • 抗原 See all GST 抗体
    GST (Glutathione S Transferase (GST))
    适用
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    Schistosoma japonicum
    宿主
    • 60
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    山羊
    克隆类型
    • 99
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    多克隆
    标记
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    This GST antibody is conjugated to Alkaline Phosphatase (AP)
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-105-200
    Supplier
    Rockland
    原理
    GST Antibody Alkaline Phosphatase Conjugated
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
    产品特性
    Synonyms: goat anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody, ALP, Alk Phos
    纯化方法
    Anti-GST Alkaline Phosphatase Conjugated antibody as prepared from monospecific antiserum by immunoaffinity chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
    过滤
    Sterile filtered
    免疫原

    Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

    Immunogen Type: Native Protein

    亚型
    IgG
    Top Product
    Discover our top product GST Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Anti-GST Antibody Alkaline Phosphatase conjugated has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry as well as other antibody based assays using alkaline phosphatase conjugates requiring lot-to-lot consistency. Optimal concentrations in these or other immunoassays should be determined by the researcher.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:1,000 - 1:5,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    有效期
    12 months
  • 抗原
    GST (Glutathione S Transferase (GST))
    别名
    GST (GST 产品)
    背景
    Background: GST Antibody is specific for the GST affinity tag. Affinity tags re appended to proteins thereby allowing them to be purified from their crude biological source using an affinity technique. Common affinity tags include glutathione-S-transferase (GST), chitin binding protein (CBP), maltose binding protein (MBP), and the poly-Histidine or HIS-tag.
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