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GST 抗体

GST 适用: Schistosoma japonicum WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
Rockland
产品编号 ABIN99861
Supplier Product No.: 100-101-200
发货至: 中国
  • 抗原 See all GST 抗体
    GST (Glutathione S Transferase (GST))
    适用
    • 95
    • 29
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    Schistosoma japonicum
    宿主
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    山羊
    克隆类型
    • 99
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    多克隆
    标记
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    This GST antibody is un-conjugated
    应用范围
    • 147
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    100-101-200
    Supplier
    Rockland
    原理
    GST Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum].
    产品特性
    Synonyms: goat anti-GST Antibody, GST, Glutathione-S-Transferase
    纯化方法
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    免疫原

    Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]

    Immunogen Type: Native Protein

    Top Product
    Discover our top product GST Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-GST has been tested by western blot and is suitable to be assayed against 1.0 μg of Glutathione-S-Transferase [Schistosoma japonicum] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:8,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:1,000 - 1:5,000

    ELISA Dilution: 1:10,000 - 1:50,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    90 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Miller, Tejada, Gazzano-Santoro: "Development of an ELISA based bridging assay as a surrogate measure of ADCC." in: Journal of immunological methods, (2012) (PubMed).

  • 抗原
    GST (Glutathione S Transferase (GST))
    别名
    GST (GST 产品)
    背景
    Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.
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