GLN1 抗体
Quick Overview for GLN1 抗体 (ABIN99838)
抗原
See all GLN1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 100-101-238
-
Supplier
- Rockland
-
原理
- Glutamine Synthetase Antibody
-
交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutamine Synthetase [Microbial].
-
产品特性
- Synonyms: Glutamine synthetase, goat anti-Glutamine Synthetase Antibody
-
纯化方法
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
-
免疫原
-
Immunogen: Glutamine Synthetase [native from brevibacterium species]
Immunogen Type: Native Protein
-
-
-
-
应用备注
-
Application Note: Anti-Glutamine Synthetase has been tested by western blot and is suitable to be assayed against 1.0 μg of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
-
浓度
- 90 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- GLN1 (Glutamine Synthetase (GLN1))
-
别名
- Glutamine Synthetase
-
背景
- Background: Catalytic activity of Glutamine Synthetase is ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
-
基因ID
- 3345165
-
UniProt
- Q79VE3
-
途径
- Positive Regulation of Peptide Hormone Secretion
抗原
-