Glucose Oxidase 抗体
Quick Overview for Glucose Oxidase 抗体 (ABIN99713)
抗原
适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-6137-0100
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Supplier
- Rockland
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原理
- Glucose Oxidase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger].
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产品特性
- Synonyms: sheep anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
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纯化方法
- Anti-GLUCOSE OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Glucose Oxidase [Aspergillus niger]
Immunogen Type: Native Protein
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亚型
- IgG
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应用备注
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Application Note: Glucose Oxidase antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) code #613-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,500 - 1:25,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Glucose Oxidase (GOD)
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别名
- Glucose Oxidase
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背景
- Background: Glucose Oxidase enzyme is widely applied for the determination of glucose in body fluids and in removing residual glucose or oxygen from foods and beverages. Furthermore, glucose oxidase-producing molds such as aspergillus and penicillium species are used for the biological production of gluconic acid.
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基因ID
- 2683500
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UniProt
- P13006
抗原
-
