Glucose Oxidase 抗体
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- 抗原 See all Glucose Oxidase (GOD) products
- Glucose Oxidase (GOD)
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适用
- Aspergillus niger
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Glucose Oxidase antibody is un-conjugated
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应用范围
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 100-4137
- Supplier
- Rockland
- 原理
- Glucose Oxidase Antibody
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Glucose Oxidase [Aspergillus niger].
- 产品特性
- Synonyms: rabbit anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody
- 纯化方法
- Anti-Glucose Oxidase Antibody was prepared from monospecific antiserum by a delipidation and defibrination.
- 免疫原
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Immunogen: Glucose Oxidase [Aspergillus niger]
Immunogen Type: Native Protein
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- 应用备注
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Application Note: Anti-Glucose Oxidase Antibody has been assayed against 1.0 μg of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Rabbit) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for Anti-Glucose Oxidase Antibody.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 90 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: None - 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Glucose Oxidase (GOD)
- 别名
- Glucose Oxidase (GOD 产品)
- 背景
- Background: Glucose Oxidase, first described in Aspergillus niger), catalyzes the oxidation of glucose into gluconic acid whilst in an aerobic environment. Glucose Oxidase is one of the most widely used biomarkers due to the large enthalpy change observed when it oxidizes glucose. Glucose oxidase can be used as a label in immunoassays when attached to antibodies and for staining tissues. In respect to glucose oxidases' ability to stain tissues, it is most commonly used to determine the levels of glucose in blood, serum, and plasma, as part of colorimetric assays. Anti-Glucose Oxidase Antibody is ideal for investigators in Immunology, Molecular Biology, and Hematology Research.
- UniProt
- P13006
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