GLB1 抗体 (HRP)
Quick Overview for GLB1 抗体 (HRP) (ABIN99529)
抗原
See all GLB1 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4336
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Supplier
- Rockland
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原理
- Beta Galactosidase Antibody Peroxidase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, and anti-Rabbit Serum as well as purified and partially purified Beta Galactosidase [E.
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产品特性
- Synonyms: rabbit anti-Beta Galactosidase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Beta Galactosidase Antibody, rabbit anti-beta gal HRP conjugated antibody
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纯化方法
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Beta Galactosidase (E.coli)
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Galactosidase, beta 1 (GLB1) antibody
GLB1 适用: 人, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1C9 unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 1-270) antibodyGLB1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 68-219) antibodyGLB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 578-677) antibodyGLB1 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 6E7 unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 317-455) antibodyGLB1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) antibodyGLB1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 192-432) antibodyGLB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 301-380) antibodyGLB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) antibodyGLB1 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Galactosidase, beta 1 (GLB1) (AA 1-677) antibodyGLB1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,000
Application Note: Anti-Beta Galactosidase Peroxidase Conjugated Antibody has been tested by ELISA and Western blot and is suitable for dot blot, immunofluorescence microscopy, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity. The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4 % paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5,000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus. A 1:1,500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ. In this particular experiment, tissue was perfused with 4 % paraformaldehyde and cryostat-cut (35 μm) to produce free-floating sections. A 1:5,000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy. A 1:5,000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos. Although a wide range of conditions was reported to be effective, a 1:10,000 dilution was noted to show no background and to be suitable for double labeling experiments. Optimal titers for other applications should be determined by the researcher.
Western Blot Dilution: 1:2,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- GLB1 (Galactosidase, beta 1 (GLB1))
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别名
- Beta Galactosidase
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背景
- Background: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations. Anti-beta Galactosidase Antibody is ideal for investigators involved in enzyme research.
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NCBI登录号
- NP_414878
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UniProt
- P00722
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途径
- Glycosaminoglycan Metabolic Process
抗原
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