FGA 抗体
Quick Overview for FGA 抗体 (ABIN99207)
抗原
See all FGA 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-101-240-0100
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Supplier
- Rockland
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原理
- Fibrinogen Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fibrinogen [Human Plasma].
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产品特性
- Synonyms: goat anti-Fibrinogen Antibody, FGA antibody, FGA protein antibody, FGB antibody, FGG antibody, Fib2 antibody, Fibrinogen A alpha polypeptide antibody, Fibrinogen A alpha polypeptide chain antibody, Fibrinogen alpha chain antibody
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纯化方法
- Anti-FIBRINOGEN is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Fibrinogen [Human Plasma]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Fibrinogen alpha Chain (FGA) (AA 124-214) antibody
FGA 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fibrinogen alpha Chain (FGA) (AA 39-615) antibodyFGA 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibrinogen alpha Chain (FGA) (AA 1-218) antibodyFGA 适用: 人 WB, ELISA, IP, PLA 宿主: 小鼠 Monoclonal 1D12 unconjugated
anti-Fibrinogen alpha Chain (FGA) (AA 34-255) antibodyFGA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fibrinogen alpha Chain (FGA) (AA 1-218) antibodyFGA 适用: 人 WB, IHC (p), PLA 宿主: 兔 Polyclonal unconjugated
anti-Fibrinogen alpha Chain (FGA) antibodyFGA 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Fibrinogen alpha Chain (FGA) (AA 61-160) antibodyFGA 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Fibrinogen alpha Chain (FGA) (AA 123-135) antibodyVerified FGA 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Fibrinogen alpha Chain (FGA) (C-Term) antibodyFGA 适用: 人, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Fibrinogen alpha Chain (FGA) (AA 1-100) antibodyFGA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500
Application Note: Anti-FIBRINOGEN has been tested in western blot and immunohistochemistry. This antibody is suitable when assayed against 1.0 μg of Fibrinogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000 - 1:100,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 10.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- FGA (Fibrinogen alpha Chain (FGA))
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别名
- FGA
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背景
- Background: Fibrinogen (factor I) is a soluble plasma glycoprotein, synthesized by the liver, that is converted by thrombin into fibrin during blood coagulation. This is achieved through processes in the coagulation cascade that activate the zymogen prothrombin to the serine protease thrombin, which is responsible for converting fibrinogen into fibrin. Fibrin is then cross linked by factor XIII to form a clot. FXIIIa stabilizes fibrin further by incorporation of the fibrinolysis inhibitors alpha-2-antiplasmin and TAFI (thrombin activatable fibrinolysis inhibitor, procarboxypeptidase B), and binding to several adhesive proteins of various cells. Both the activation of Factor XIII by thrombin and plasminogen activator (t-PA) are catalyzed by fibrin. Fibrin specifically binds the activated coagulation factors factor Xa and thrombin and entraps them in the network of fibers, thus functioning as a temporary inhibitor of these enzymes, which stay active and can be released during fibrinolysis. Recent research has shown that fibrin plays a key role in the inflammatory response. Anti-Fibrinogen (Human Plasma) is ideal for Serum Protein Component research.
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基因ID
- 2243
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NCBI登录号
- NP_000499
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UniProt
- P02671
抗原
-
