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FGA 抗体

FGA 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
Rockland
产品编号 ABIN99199
Supplier Product No.: 100-101-240
发货至: 中国
  • 抗原 See all FGA 抗体
    FGA (Fibrinogen alpha Chain (FGA))
    适用
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    宿主
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    山羊
    克隆类型
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    多克隆
    标记
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    This FGA antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Supplier Product No.
    100-101-240
    Supplier
    Rockland
    原理
    Fibrinogen Antibody
    交叉反应 (详细)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Fibrinogen [Human Plasma].
    产品特性
    Synonyms: goat anti-Fibrinogen Antibody, FGA antibody, FGA protein antibody, FGB antibody, FGG antibody, Fib2 antibody, Fibrinogen A alpha polypeptide antibody, Fibrinogen A alpha polypeptide chain antibody, Fibrinogen alpha chain antibody
    纯化方法
    This product was prepared from monospecific antiserum by a delipidation and defibrination.
    免疫原

    Immunogen: Fibrinogen [Human Plasma]

    Immunogen Type: Native Protein

    Top Product
    Discover our top product FGA Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:500

    Application Note: Anti-Fibrinogen has been tested by western blot and immunohistochemistry and is suitable to be assayed against 1.0 μg of Fibrinogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:2,000 - 1:10,000

    ELISA Dilution: 1:20,000 - 1:100,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    90 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    FGA (Fibrinogen alpha Chain (FGA))
    别名
    FGA (FGA 产品)
    背景
    Background: Fibrinogen is cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA), fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, it functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. It was originally thought to be essential for platelet aggregation, based on in vitro studies using anti-coagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. It enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. It may also facilitate the immune response via both innate and T-cell mediated pathways.
    基因ID
    2243
    NCBI登录号
    NP_000499
    UniProt
    P02671
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