CES3 抗体
Quick Overview for CES3 抗体 (ABIN98993)
抗原
See all CES3 抗体适用
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 200-4169-0100
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Supplier
- Rockland
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原理
- Esterase Antibody
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Esterase [Porcine Liver].
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产品特性
- Synonyms: rabbit anti-Esterase Antibody, Liver carboxylesterase, Proline-beta-naphthylamidase, Retinyl ester hydrolase
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纯化方法
- Anti-Esterase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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免疫原
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Immunogen: Esterase [Porcine Liver]
Immunogen Type: Native Protein
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亚型
- IgG
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anti-Carboxylesterase 3 (CES3) (AA 65-233) antibody
CES3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) antibodyCES3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) (AA 1-568) antibodyCES3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) (AA 269-568) antibodyCES3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Carboxylesterase 3 (CES3) (C-Term) antibodyCES3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Application Note: Anti-Esterase is suitable for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:10,000 - 1:50,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- CES3 (Carboxylesterase 3 (CES3))
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别名
- CES3
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背景
- Background: Esterase is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It is active towards triacylglycerides containing short-chain fatty acids from C2 to C6, and 13-monoacylglycerols containing fatty acids from C2 to C12, and inactive on long-chain triacylglycerols and diacylglycerol. it hydrolyzes aromatic and alkyl esters and vitamin A acetate. The hydrolysis rate depends upon the amino acid promoiety and the esterification site of the prodrug. Aromatic promoieties are favored, highest rates are observed with phenylalanyl progdrugs, hydrolysis of valyl and isoleucyl prodrugs is less efficient. With floxuridine prodrugs, activity is higher on 5' monoesters than on 3' monoesters, while with gemcitabine prodrugs, activity is higher on 3' monoesters than on 5' monoesters.
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基因ID
- 100626873, 343791015
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UniProt
- Q29550
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途径
- Monocarboxylic Acid Catabolic Process
抗原
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