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ZAP70 抗体 (Internal Region)

Verified ZAP70 适用: 人 WB, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN987274
发货至: 中国
  • 抗原 See all ZAP70 抗体
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    抗原表位
    • 35
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    Internal Region
    适用
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    宿主
    • 205
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    山羊
    克隆类型
    • 197
    • 74
    多克隆
    标记
    • 136
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    This ZAP70 antibody is un-conjugated
    应用范围
    • 194
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    原理
    ZAP70
    序列
    SDGYTPEPAR ITSPD
    特异性
    This antibody is expected to recognize isoform 1 (NP_001070.2) only.
    交叉反应
    Cow, 犬, 人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2.
    亚型
    IgG
    Top Product
    Discover our top product ZAP70 Primary Antibody
  • 应用备注

    Western Blot: Approx 70 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9 kDa according to NP_001070.2). Recommended concentration: 0.3-1 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer).

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (201

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    别名
    ZAP70 (ZAP70 产品)
    别名
    SRK antibody, STCD antibody, STD antibody, TZK antibody, ZAP-70 antibody, 70kDa antibody, AI327364 antibody, Srk antibody, mrtle antibody, mur antibody, zap70 antibody, MGC80670 antibody, MGC88962 antibody, ZAP70 antibody, zgc:110383 antibody, zeta chain of T-cell receptor associated protein kinase 70 antibody, putative tyrosine-protein kinase zap-70 antibody, zeta-chain (TCR) associated protein kinase antibody, zeta chain of T cell receptor associated protein kinase 70 antibody, zeta-chain (TCR) associated protein kinase 70kDa antibody, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antibody, zeta chain of T cell receptor associated protein kinase 70kDa antibody, tyrosine-protein kinase ZAP-70 antibody, ZAP70 antibody, Smp_149470 antibody, Zap70 antibody, zap70.L antibody, zap70 antibody, LOC707555 antibody, LOC100342021 antibody, LOC100390922 antibody, LOC100542877 antibody
    背景
    ZAP70, zeta-chain (TCR) associated protein kinase 70 kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD
    基因ID
    7535, 22637, 301348
    NCBI登录号
    NP_001070
    途径
    TCR Signaling, Ubiquitin Proteasome Pathway
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