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Cyclin B1 抗体

This anti-Cyclin B1 antibody is a 兔 多克隆 antibody detecting Cyclin B1 in WB, IHC, ELISA 和 IP. Suitable for 人. This Primary Antibody has been cited in 1 publication.
Rockland
产品编号 ABIN97948
Supplier Product No.: 100-401-152
发货至: 中国

Quick Overview for Cyclin B1 抗体 (ABIN97948)

抗原

See all Cyclin B1 (CCNB1) 抗体
Cyclin B1 (CCNB1)

适用

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宿主

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克隆类型

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多克隆

标记

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This Cyclin B1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
  • Supplier Product No.

    100-401-152

    Supplier

    Rockland

    原理

    Cyclin B1 Antibody

    交叉反应 (详细)

    Antiserum will specifically react with a 55-62 kDa Cyclin B1 protein from human, rat and mouse tissue.

    产品特性

    Synonyms: rabbit anti-cyclin B1 antibody, G2/mitotic-specific cyclin-B1

    纯化方法

    This product was prepared from monospecific antiserum by delipidation and defibrination.

    过滤

    Sterile filtered

    免疫原

    Immunogen: Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.

    Immunogen Type: Native Protein

  • 应用备注

    Immunohistochemistry Dilution: 1:500

    Application Note: Anti-Cyclin B1 antibody has been tested by western blot and immunohistochemistry and is suitable for use in ELISA, immunoblotting, immunoprecipitation, immunohistochemistry, and other immunological methods requiring high titer and specificity.   Specific conditions for reactivity and detection of Cyclin B1 should be optimized by the end user.  Expect a band approximately 55-66 kDa in size corresponding to Cyclin B1 by western blotting in the appropriate cell lysate or extract. H23 cells may be used as a positive control.

    Western Blot Dilution: 1:500 - 1:1,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    80 mg/mL

    缓冲液

    Buffer: None

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

    有效期

    12 months
  • Wetzel-Strong, Li, Klein, Nishikimi, Caron: "Epicardial-derived adrenomedullin drives cardiac hyperplasia during embryogenesis." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 243, Issue 2, pp. 243-56, (2014) (PubMed).

  • 抗原

    Cyclin B1 (CCNB1)

    别名

    CCNB1

    背景

    Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

    基因ID

    891, 14327896

    UniProt

    P14635

    途径

    Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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