Cyanine 抗体 (Biotin)
Quick Overview for Cyanine 抗体 (Biotin) (ABIN97920)
抗原
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-606-209
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Supplier
- Rockland
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原理
- Cyanine Antibody Biotin Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep Serum and cyanine conjugated IgG.
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产品特性
- Synonyms: sheep anti Cyanine Antibody Biotin Conjugated
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
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Immunogen: A mixture of Cy3 and Cy5 conjugated KLH
Immunogen Type: Other
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亚型
- IgG
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anti-Cyanine antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB, DB 宿主: 绵羊 Polyclonal Alkaline Phosphatase (AP)
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应用备注
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Application Note: This product has been assayed against 1.0 μg of Cyanine IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:8,000-1:40,000
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Cyanine
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物质类
- Chemical
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背景
- Background: Cyanine is a synthetic family of dyes that can cover the spectrum from infrared to ultraviolent light. Cyanine is useful in protein and nucleic acid labeling. This Anti-Cyanine Antibody is conjugated to Biotin. Anti-Cyanine (sheep) antibody is ideal for investigators in Cell Biology, Immunology, and Microbiology research.
抗原
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