Cyanine 抗体 (Alkaline Phosphatase (AP))
Quick Overview for Cyanine 抗体 (Alkaline Phosphatase (AP)) (ABIN97919)
抗原
宿主
克隆类型
标记
应用范围
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Supplier Product No.
- 600-605-209
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Supplier
- Rockland
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原理
- Cyanine Antibody Alkaline Phosphatase Conjugated
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交叉反应 （详细）
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Sheep Serum and cyanine conjugated IgG.
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产品特性
- Synonyms: sheep anti-Cyanine Antibody alkaline phosphatase Conjugation, alk phos conjugated sheep anti-Cyanine Antibody
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纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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过滤
- Sterile filtered
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免疫原
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Immunogen: A mixture of Cy3 and Cy5 conjugated KLH
Immunogen Type: Other
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Cyanine Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and dot blot. This product has been assayed against 1.0 μg of Cyanine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:700 to 1:2,800 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:2,000 - 1:10,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.4 mg/mL
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缓冲液
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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有效期
- 12 months
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- Cyanine
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物质类
- Chemical
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背景
- Background: Cyanine is a synthetic family of dyes that can cover the spectrum from infrared to ultraviolent light. Cyanine is useful in protein and nucleic acid labeling. This Anti-Cyanine Antibody is conjugated to Alkaline Phosphatase. Anti-Cyanine (sheep) antibody is ideal for investigators in Cell Biology, Immunology, and Microbiology research.
抗原
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