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Glypican 3 抗体

Name: Glypican 3 抗体
Brand: antibodies-online
SKU: ABIN969523
Availability: OutOfStock

This anti-Glypican 3 antibody is a 小鼠单克隆 antibody detecting Glypican 3 in FACS, WB, IHC, ELISA 和 ICC. Suitable for 人, 大鼠和小鼠. This Primary Antibody has been cited in 2+ publications.

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GPC3 mouse mAb with DAB staining. (Glypican 3 抗体)

IHC

Flow cytometric analysis of Jurkat cells using GPC3 mouse mAb (green) and negative control (red). (Glypican 3 抗体)

FACS

Immunofluorescence analysis of HeLa cells using GPC3 mouse mAb (green). (Glypican 3 抗体)

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPC3 mouse mAb with DAB staining. (Glypican 3 抗体)

IHC

Western blot analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate. (Glypican 3 抗体)

Western blot analysis using GPC3 mAb against human GPC3 (AA: 55-200) recombinant protein. (Glypican 3 抗体)

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (Glypican 3 抗体)

ELISA

7 images

(2 references)

产品编号 ABIN969523

发货至: 中国

Quick Overview for Glypican 3 抗体 (ABIN969523)

抗原

See all Glypican 3 (GPC3) 抗体

Glypican 3 (GPC3)

适用

人, 大鼠, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This Glypican 3 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

克隆位点

9C2

原理

GPC3 Antibody

纯化方法

Purified antibody

免疫原

Purified recombinant fragment of human GPC3 expressed in E. Coli.

亚型

IgG1
anti-Glypican 3 (GPC3) (AA 344-559) antibody
GPC3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Glypican 3 (GPC3) antibody
GPC3 适用: 人, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal GPC3-863 unconjugated

anti-Glypican 3 (GPC3) (AA 55-200) antibody
GPC3 适用: 人 IF, FACS, WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 9C2 unconjugated

anti-Glypican 3 (GPC3) (AA 359-554) antibody
GPC3 适用: 人 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2C7E9 unconjugated

anti-Glypican 3 (GPC3) (AA 55-200) antibody
GPC3 适用: 人, 小鼠 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 2A2C3 unconjugated

anti-Glypican 3 (GPC3) antibody
GPC3 适用: 人, 大鼠 IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal rGPC3-863 unconjugated Recombinant Antibody

anti-Glypican 3 (GPC3) (AA 511-580) antibody
GPC3 适用: 人 IF, FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal 1G12 unconjugated

anti-Glypican 3 (GPC3) (AA 359-554) antibody
GPC3 适用: 人, 大鼠 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 4D5B1 unconjugated

anti-Glypican 3 (GPC3) antibody
GPC3 适用: 人, 小鼠 IF, WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 9C2 unconjugated

anti-Glypican 3 (GPC3) (AA 343-559) antibody
GPC3 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/200 - 1/1000

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Zittermann, Capurro, Shi, Filmus: "Soluble glypican 3 inhibits the growth of hepatocellular carcinoma in vitro and in vivo." in: International journal of cancer. Journal international du cancer, Vol. 126, Issue 6, pp. 1291-301, (2010) (PubMed).

Takai, Ashihara, Ishiguro, Terashima, Watanabe, Kato, Suzuki: "Involvement of glypican-3 in the recruitment of M2-polarized tumor-associated macrophages in hepatocellular carcinoma." in: Cancer biology & therapy, Vol. 8, Issue 24, pp. 2329-38, (2010) (PubMed).
抗原

Glypican 3 (GPC3)

别名

GPC3

背景

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.

分子量

65.5 kDa

基因ID

2719

UniProt

P51654

途径

Glycosaminoglycan Metabolic Process