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Vimentin 抗体 (AA 2-466)

Name: Vimentin 抗体 (AA 2-466)
Brand: antibodies-online
SKU: ABIN969454
Availability: OutOfStock

VIM 适用: 人, 猴 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4F2E9 unconjugated

Western blot analysis using Vimentin mouse mAb against Hela (1), COS (2), HEK293 (3) and U20S (4) cell lysate. (Vimentin 抗体 (AA 2-466))

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, showing cytoplasmic localization using Vimentin mouse mAb with DAB staining. (Vimentin 抗体 (AA 2-466))

IHC

2 images

(2 references)

产品编号 ABIN969454

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
抗原表位
32

18

16

16

15

14

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-466
适用
332

126

125

61

58

42

42

36

29

21

20

13

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
人, 猴
宿主
190

128

9

6

4

4

2
小鼠
Compatible Secondaries
克隆类型
198

144
单克隆
标记
199

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
281

132

99

97

95

65

49

40

39

28

23

20

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA

纯化方法

purified

免疫原

Purified recombinant fragment of Vimentin (aa2-466) expressed in E. coli.

克隆位点

4F2E9

亚型

IgG1

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VIM 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C8 unconjugated

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VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

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VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000

限制

仅限研究用
状态

Liquid

缓冲液

Ascitic fluid containing 0.03 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

4°C, -20°C for long term storage
Yates, Zetterberg, Rajeev, Reiss, Rittling: "Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta." in: Experimental cell research, Vol. 313, Issue 17, pp. 3718-28, (2007) (PubMed).

Gilles, Polette, Mestdagt, Nawrocki-Raby, Ruggeri, Birembaut, Foidart: "Transactivation of vimentin by beta-catenin in human breast cancer cells." in: Cancer research, Vol. 63, Issue 10, pp. 2658-64, (2003) (PubMed).
抗原

Vimentin (VIM)

别名

Vimentin (VIM 产品)

别名

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

背景

Description: Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Aliases: VIM

分子量

54 kDa

基因ID

7431

HGNC

7431

途径

Caspase Cascade in Apoptosis