NPT 抗体
Our Local Distributor
北京 101111
Quick Overview for NPT 抗体 (ABIN969318)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- NPT Antibody
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纯化方法
- Ascitic fluid
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免疫原
- Purified recombinant fragment of NPT expressed in E. Coli.
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亚型
- IgG2a
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应用备注
- ELISA: 1/10000
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Ascitic fluid containing 0.03 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
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: "Reduction of poliovirus 1, bacteriophages, Salmonella montevideo, and Escherichia coli O157:H7 on strawberries by physical and disinfectant washes." in: Journal of food protection, Vol. 66, Issue 2, pp. 188-93, (2003) (PubMed).
: "Transgenic plants and biosafety: science, misconceptions and public perceptions." in: BioTechniques, Vol. 29, Issue 4, pp. 832-6, 838-43, (2001) (PubMed).
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: "Reduction of poliovirus 1, bacteriophages, Salmonella montevideo, and Escherichia coli O157:H7 on strawberries by physical and disinfectant washes." in: Journal of food protection, Vol. 66, Issue 2, pp. 188-93, (2003) (PubMed).
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- NPT
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背景
- Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.
抗原
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