SPTBN1 抗体 (AA 2101-2189)
(3 references)Quick Overview for SPTBN1 抗体 (AA 2101-2189) (ABIN968882)
抗原
See all SPTBN1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 2101-2189
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交叉反应
- 犬, 小鼠, 大鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human beta-Spectrin II aa. 2101-2189
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亚型
- IgG1
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anti-Spectrin Beta, Non-Erythrocytic 1 (SPTBN1) (AA 2096-2256) antibody
SPTBN1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Spectrin Beta, Non-Erythrocytic 1 (SPTBN1) (N-Term) antibodySPTBN1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Spectrin Beta, Non-Erythrocytic 1 (SPTBN1) (AA 2063-2097) antibodySPTBN1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB54579 unconjugated
anti-Spectrin Beta, Non-Erythrocytic 1 (SPTBN1) antibodySPTBN1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Spectrin Beta, Non-Erythrocytic 1 (SPTBN1) (AA 2100-2200) antibodySPTBN1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- For western blot analysis use at 1:1000.
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说明
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Related Products: ABIN968537
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "Tyrosine phosphorylation regulates alpha II spectrin cleavage by calpain." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3527-36, (2002) (PubMed).
: "Characterization of human brain cDNA encoding the general isoform of beta-spectrin." in: The Journal of biological chemistry, Vol. 267, Issue 26, pp. 18715-22, (1992) (PubMed).
: "Generation of diversity in nonerythroid spectrins. Multiple polypeptides are predicted by sequence analysis of cDNAs encompassing the coding region of human nonerythroid alpha-spectrin." in: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4427-33, (1990) (PubMed).
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: "Tyrosine phosphorylation regulates alpha II spectrin cleavage by calpain." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3527-36, (2002) (PubMed).
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- SPTBN1 (Spectrin Beta, Non-Erythrocytic 1 (SPTBN1))
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别名
- beta-Spectrin II
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背景
- Spectrins are central components of the cytoskeleton that form a scaffold below the plasma membrane. Spectrins contain two subunits, alpha and ß, which intertwine to form heterodimers that can self associate into elongated tetramers. alpha-spectin I and ß-spectrin I form heterodimers in red blood cells, while nonerythroid mammalian cells contain heterodimers of alpha-spectin I and II with ß-spectrin I to V. The structure of spectrins includes a succession of triple-helical repeats alongwith various domains, such as SH3 domain, EF hands, PH domains, and binding domains for ankyrin, actin, band 4.1, and calmodulin. alpha-spectrin II is a widely expressed non-erythroid alpha-spectrin that contains an SH3 domain, a calmodulin binding site, and two cleavage sites for proteases, such as calpains and caspase-3. ß-spectrin II is a widely expressed non-erythroid ß-spectrin that contains a C-terminal region that interacts with alpha-spectrins and a PH domain. alpha-spectrin II and ß-spectrin II, like many other spectrins, can form heterodimers that can self associate into tetramers, as well as interact with Band 4.1, F-actin, and other proteins near the plasma membrane. This scaffold of cytoskeletal and plasma membrane proteins is critical for the maintenance of cell structure. This antibody is routinely tested by the Western blot analysis.
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分子量
- 280 kDa
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途径
- Regulation of Actin Filament Polymerization, Protein targeting to Nucleus
抗原
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