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PPP1R12A 抗体 (AA 723-840)

Name: PPP1R12A 抗体 (AA 723-840)
SKU: ABIN968768
Availability: OutOfStock

This anti-PPP1R12A antibody is a 小鼠单克隆 antibody detecting PPP1R12A in WB. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 3+ publications.

Western blot analysis of MYPT1 on a rat cerebrum lysate. (PPP1R12A 抗体 (AA 723-840))

1 image

(3 references)

产品编号 ABIN968768

发货至: 中国

Datasheet as PDF

Quick Overview for PPP1R12A 抗体 (AA 723-840) (ABIN968768)

抗原

See all PPP1R12A 抗体

PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This PPP1R12A antibody is un-conjugated

应用范围

Western Blotting (WB)

克隆位点

20-MYPT1

Discover our top product PPP1R12A Primary Antibody

抗原表位
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16

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2

2

2

2

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1

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1

1
AA 723-840

交叉反应

人, 小鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Rat MYPT1 aa. 723-840

亚型

IgG1
anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibody
Verified PPP1R12A 适用: 人 IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 1-200) antibody
PPP1R12A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 1-40), (N-Term) antibody
PPP1R12A 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (C-Term) antibody
PPP1R12A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr696) antibody
PPP1R12A 适用: 人, 大鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated

anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibody
PPP1R12A 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 481-514), (Ser507) antibody
PPP1R12A 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB56872 unconjugated

anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pThr853) antibody
PPP1R12A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 710-970) antibody
PPP1R12A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (pSer696) antibody
PPP1R12A 适用: 人, 小鼠, 大鼠, 小鸡, 斑马鱼, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

说明

Related Products: ABIN968545, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Khatri, Joyce, Brozovich, Fisher: "Role of myosin phosphatase isoforms in cGMP-mediated smooth muscle relaxation." in: The Journal of biological chemistry, Vol. 276, Issue 40, pp. 37250-7, (2001) (PubMed).

Kimura, Ito, Amano, Chihara, Fukata, Nakafuku, Yamamori, Feng, Nakano, Okawa, Iwamatsu, Kaibuchi: "Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)" in: Science (New York, N.Y.), Vol. 273, Issue 5272, pp. 245-8, (1996) (PubMed).

Chen, Chen, Alessi, Campbell, Shanahan, Cohen, Cohen: "Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M." in: FEBS letters, Vol. 356, Issue 1, pp. 51-5, (1995) (PubMed).
抗原

PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

别名

MYPT1

背景

Protein phosphatase-1 (PP1) is a major serine/threonine phosphatase that is involved in various cellular processes in eukaryotic cells. The PP1 catalytic subunit may be directed to particular subcellular locations by targeting subunits, which can modify the substrate specificity of the enzyme. The PP1 enzyme associated with the myofibrils of striated and smooth muscle forms a complex with Myosin Phosphatase Targeting subunit 1 (MYPT1). MYPT1 contains seven ankyrin repeats in the N-terminal region, and a leucine zipper (LZ) motif in the C-terminus. Alternative splicing of the MYPT1 mRNA leads to expression of a truncated MYPT1 that lacks the leucine zipper region. The expression of MYPT1 splice variants correlate with a smooth muscle phenotype. In the tonic contracting chicken aorta, only the full length MYPT1 is expressed, while in the phasic contracting chicken gizzard, only the truncated MYPT1 is expressed. The leucine zipper motif may be required for association with cGMP-dependent protein kinase I. Thus, MYPT1 may direct not only PP1 enzymatic activity toward myosin, but also may regulate the cGMP responsiveness of PP1 through expression of alternative splice variants.
Synonyms: Myosin Phosphatase Targeting subunit 1

分子量

130 kDa

途径

M Phase