GBF1 抗体 (AA 1266-1379)
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北京 101111
Quick Overview for GBF1 抗体 (AA 1266-1379) (ABIN968745)
抗原
See all GBF1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1266-1379
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交叉反应
- 小鼠, 大鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human GBF1 aa. 1266-1379
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亚型
- IgG1
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anti-Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1) (AA 1-85) antibody
GBF1 适用: 人 IF, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1) (AA 101-209) antibodyGBF1 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 4B2 unconjugated
anti-Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1) (N-Term) antibodyGBF1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1) (AA 1-85) antibodyGBF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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说明
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Related Products: ABIN968535, ABIN967389
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "GBF1: A novel Golgi-associated BFA-resistant guanine nucleotide exchange factor that displays specificity for ADP-ribosylation factor 5." in: The Journal of cell biology, Vol. 146, Issue 1, pp. 71-84, (1999) (PubMed).
: "Human GBF1 is a ubiquitously expressed gene of the sec7 domain family mapping to 10q24." in: Genomics, Vol. 54, Issue 2, pp. 323-7, (1999) (PubMed).
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: "GBF1: A novel Golgi-associated BFA-resistant guanine nucleotide exchange factor that displays specificity for ADP-ribosylation factor 5." in: The Journal of cell biology, Vol. 146, Issue 1, pp. 71-84, (1999) (PubMed).
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- GBF1 (Golgi Brefeldin A Resistant Guanine Nucleotide Exchange Factor 1 (GBF1))
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别名
- GBF1
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背景
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The Golgi apparatus is a complex and dynamic organelle that functions in protein sorting and modification. Numerous structural and regulatory proteins are involved in the budding, docking, and fusion of Golgi-directed vesicles. Golgi-specific Brefeldin A (BFA)-resistance Factor (GBF1) is a guanine nucleotide exchange factor that contains a centrally located Sec7 domain, and a proline-rich region at the C-terminus. The Sec7 domain is commonly found in a variety of secretory proteins, and is required for activation of ARFs. GBF1 mRNA is ubiquitously expressed, and GBF1 protein localizes to the Golgi, as well as to perinuclear structures that contain COPI. BFA is a fungal heterocyclic lactone that disrupts membrane recruitment of ARFs. This action blocks protein secretion, and changes the morphology of various organelles, such as the Golgi. Overexpression of GBF1 suppresses BFA-induced changes in Golgi morphology, ARF activation, and coat protein recruitment. GBF1 interacts preferentially with ARF5 in the presence of Mg2+. Thus, GBF1 is a Golgi-specific guanine nucleotide exchange factor that activates ARFs through a mechanism that interferes with BFA action.
Synonyms: Golgi-specific Brefeldin A (BFA)-resistance Factor 1 -
分子量
- 206 kDa
抗原
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