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Synaptophysin 抗体 (AA 205-306)

Name: Synaptophysin 抗体 (AA 205-306)
SKU: ABIN968694
Availability: OutOfStock

SYP 适用: 人, 小鼠, 大鼠 WB, IF, BI 宿主: 小鼠 Monoclonal 2-Synaptophysin unconjugated

Western blot analysis of Synaptophysin on a rat cerebrum lysate.

Immunofluorescence (IF) image for anti-Synaptophysin (SYP) (AA 205-306) antibody (ABIN968694)

Western Blotting (WB) image for anti-Synaptophysin (SYP) (AA 205-306) antibody (ABIN968694)

Immunofluoresence staining of PC12 cells (rat neuroblastoma, ATCC CRL 1721).

4 images

(3 references)

产品编号 ABIN968694

发货至: 中国

Datasheet as PDF

抗原 See all Synaptophysin (SYP) 抗体

Synaptophysin (SYP)
抗原表位
13

6

6

5

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 205-306
适用
74

55

54

10

10

6

6

3

3

2

2

2

1

1

1

1
人, 小鼠, 大鼠
宿主
53

34

3

1
小鼠
Compatible Secondaries
克隆类型
50

41
单克隆
标记
69

3

2

2

2

2

2

2

2

2

2

1
This Synaptophysin antibody is un-conjugated
应用范围
69

42

28

21

20

15

15

6

5

3

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

交叉反应

人, 小鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Rat Synaptophysin aa. 205-306

克隆位点

2-Synaptophysin

亚型

IgG1

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anti-Synaptophysin (SYP) (AA 150-250) antibody
SYP 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Synaptophysin (SYP) (C-Term) antibody
SYP 适用: 人 WB, IF, IHC (p), ICC, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Synaptophysin (SYP) (AA 225-253), (C-Term) antibody
SYP 适用: 人, 小鼠 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB32966 unconjugated

anti-Synaptophysin (SYP) (AA 225-253), (C-Term) antibody
SYP 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB32966 unconjugated

anti-Synaptophysin (SYP) (Internal Region) antibody
SYP 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Synaptophysin (SYP) antibody
SYP 适用: 人 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G3 unconjugated

anti-Synaptophysin (SYP) antibody
SYP 适用: 人, 犬 WB, IF 宿主: 小鼠 Monoclonal 5H2 unconjugated

anti-Synaptophysin (SYP) (N-Term) antibody
SYP 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Synaptophysin (SYP) antibody
Microarray verified SYP 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB551901 unconjugated

anti-Synaptophysin (SYP) (AA 1-313) antibody
SYP 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B3 unconjugated

说明

Related Products: ABIN968545, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20°C.
Daly, Sugimori, Moreira, Ziff, Llinás: "Synaptophysin regulates clathrin-independent endocytosis of synaptic vesicles." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 11, pp. 6120-5, (2000) (PubMed).

Thiele, Hannah, Fahrenholz, Huttner: "Cholesterol binds to synaptophysin and is required for biogenesis of synaptic vesicles." in: Nature cell biology, Vol. 2, Issue 1, pp. 42-9, (2000) (PubMed).

Leube, Kaiser, Seiter, Zimbelmann, Franke, Rehm, Knaus, Prior, Betz, Reinke: "Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA." in: The EMBO journal, Vol. 6, Issue 11, pp. 3261-8, (1988) (PubMed).
抗原

Synaptophysin (SYP)

别名

Synaptophysin (SYP 产品)

别名

syp-B antibody, xsypii antibody, MGC86267 antibody, MRXSYP antibody, A230093K24Rik antibody, AI848995 antibody, Syn antibody, p38 antibody, Syp1 antibody, mrxsyp antibody, syp antibody, xsypi antibody, synaptophysin S homeolog antibody, synaptophysin antibody, synaptophysin L homeolog antibody, synaptophysin a antibody, syp.S antibody, SYP antibody, syp antibody, Syp antibody, syp.L antibody, sypa antibody

背景

Synaptophysin is a major integral membrane glycoprotein that was isolated from the presynaptic vesicles of neurons. The structure of synaptophysin includes four transmembrane domains (TMD), an N-temrinal glycosylation site, nine repeats of basic tetrapeptide motifs (GYGP), multiple collagenase sensitive sites (XGP), and several collagen-type tripeptides (GXX). Synaptophysin mRNA is expressed in nervous tissues, as well as many neuroendocrine tumor cell lines. In PC12 cells and brain synaptic vesicles, synaptophysin binds cholesterol, and cholesterol depletion blocks the biogenesis of synaptic-like microvesicles. In addition, synaptophysin binds dynamin in a Ca2+-dependent manner, and injection of peptides that disrupt this interaction decreases neurotransmitter release during high-frequency stimulation of squid neurons. This decrease in neurotransmitter release results from inhibition of synaptophysin-dependent vesicle retrieval. Thus, synaptophysin may have important roles in the biogenesis and recycling of synaptic vesicles. Synaptophysin is an integral membrane glycoprotein with a calculated molecular weight of 33 kD, but has been reported to be observed at 38-42 kD due to glycosylation. This antibody is routinely tested by western blot analysis.

分子量

38 kDa

途径

Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity