Espin 抗体 (AA 458-580)
(3 references)-
- 抗原 See all Espin (ESPN) 抗体
- Espin (ESPN)
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抗原表位
- AA 458-580
- 适用
- 人, 小鼠, 大鼠, 小鸡
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Espin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 交叉反应
- 人, 小鼠, 小鸡
- 产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Rat espin aa. 458-580
- 克隆位点
- 31-Espin
- 亚型
- IgG2a
- Top Product
- Discover our top product ESPN Primary Antibody
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anti-Espin (ESPN) (AA 17-45), (N-Term) antibody
ESPN 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB20951 unconjugated
anti-Espin (ESPN) (AA 585-854) antibodyESPN 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Espin (ESPN) antibodyESPN 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 说明
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Related Products: ABIN967389
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 250 μg/mL
- 缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store undiluted at -20°C.
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: "Espin contains an additional actin-binding site in its N terminus and is a major actin-bundling protein of the Sertoli cell-spermatid ectoplasmic specialization junctional plaque." in: Molecular biology of the cell, Vol. 10, Issue 12, pp. 4327-39, (2000) (PubMed).
: "Small espin: a third actin-bundling protein and potential forked protein ortholog in brush border microvilli." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 107-19, (1998) (PubMed).
: "Identification and characterization of espin, an actin-binding protein localized to the F-actin-rich junctional plaques of Sertoli cell ectoplasmic specializations." in: Journal of cell science, Vol. 109 ( Pt 6), pp. 1229-39, (1997) (PubMed).
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: "Espin contains an additional actin-binding site in its N terminus and is a major actin-bundling protein of the Sertoli cell-spermatid ectoplasmic specialization junctional plaque." in: Molecular biology of the cell, Vol. 10, Issue 12, pp. 4327-39, (2000) (PubMed).
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- 抗原
- Espin (ESPN)
- 别名
- Espin (ESPN 产品)
- 别名
- DFNB36 antibody, je antibody, Je antibody, espin antibody, ESPN antibody, TVAG_100530 antibody, LOAG_01035 antibody, espn antibody, Espn antibody
- 背景
- Actin-binding proteins regulate the polymerization and depolymerization of actin, connect actin-based structures to membranes and to other cytoskeletal elements, power the movement of actin filaments, and cross-link actin filaments into bundles. Espins are actin binding and bundling proteins. The two isoforms of espin are the 30 kDa small espin found in brush border cells and the 110 kDa espin found in testis. Espin contains eight ankyrin repeats in the N-terminal region, two proline-rich peptides, an ATP/GTP binding P-loop domain, and a C-terminal actin bundling domain. Small espin is composed of the C-terminal actin bundling domain and a unique region at the N-terminus. Espin binds to actin with a higher affinity than small espin and is more efficient at actin bundling. During spermiogenesis, espin accumulates, along with forming parallel actin bundles, at the ectoplasmic specialization. These actin bundles anchor and position the spermatid within the seminiferous epithelium. Other actin binding proteins, such as alpha-actinin, vinculin, and fimbrin, have also been implicated in the formation of ectoplasmic specialization, however espin appears to function specifically in the testes.
- 分子量
- 110 kDa
- 途径
- Sensory Perception of Sound
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