p62 Lck Ligand (AA 257-437) 抗体
This 小鼠 单克隆 antibody specifically detects p62 Lck Ligand in IF, IP, IHC 和 WB. It exhibits reactivity toward 人, 小鼠 和 大鼠. It has been mentioned in 2+ publications
(2 references)
产品编号 ABIN968203
发货至:
中国
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Quick Overview for p62 Lck Ligand (AA 257-437) 抗体 (ABIN968203)
抗原
p62 Lck Ligand
适用
人, 小鼠, 大鼠
宿主
小鼠
克隆类型
单克隆
应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
克隆位点
3-P62 LCK LIGAND
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抗原表位
- AA 257-437
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交叉反应
- 小鼠, 大鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human p62 lck ligand aa. 257-437
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亚型
- IgG1
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说明
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Related Products: ABIN968551, ABIN967389
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20°C.
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: "The adapter protein ZIP binds Grb14 and regulates its inhibitory action on insulin signaling by recruiting protein kinase Czeta." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 6959-70, (2002) (PubMed).
: "Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 12, pp. 6191-6, (1997) (PubMed).
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: "The adapter protein ZIP binds Grb14 and regulates its inhibitory action on insulin signaling by recruiting protein kinase Czeta." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 6959-70, (2002) (PubMed).
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- p62 Lck Ligand
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背景
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P62 lck ligand (zeta-interacting protein (ZIP)) is a cytoplasmic protein that binds to the SH2 domain of lck (a T cell src tyrosine kinase) in the absence of a phosphotyrosine in either protein. The ubiquitously expressed p62 lck ligand contains a cysteine rich region that is similar to a zinc finger domain, a G protein binding region, a PEST sequence, and several phosphorylation sites. Deletion of the p62 lck ligand N-terminal domain has been reported to abrogate its binding to lck. However, mutation of the tyrosine did not have an effect. In addition, p62 lck ligand binds to the pseudosubstrate region of the PKCzeta catalytic domain. In turn, PKCzeta phosphorylates p62. p62 lck ligand binds to the dimerization region of PKCzeta, thereby inhibiting PKCzeta-PKCzeta interaction. This suggests that p62 lck ligand may compete with PKCzeta. However, it requires PKCzeta for proper subcellular localization. These data suggest that p62 lck ligand may be part of the protein bridge that links PKCzeta to the tyrosine kinases involved in signaling pathways. This antibody is routinely tested by western blot analysis.
Synonyms: Zeta Interacting Protein (ZIP) -
分子量
- 62 kDa
抗原
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