CPE 抗体 (AA 49-200)
(4 references)Quick Overview for CPE 抗体 (AA 49-200) (ABIN968159)
抗原
See all CPE 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 49-200
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交叉反应
- 大鼠, 小鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human Carboxypeptidase E aa. 49-200
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亚型
- IgG1
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anti-Carboxypeptidase E (CPE) (AA 318-476) antibody
CPE 适用: 人 WB, ELISA, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (Internal Region) antibodyCPE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) antibodyCPE 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 36-85) antibodyCPE 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 兔, 斑马鱼, Bat, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (C-Term) antibodyCPE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 201-300) antibodyCPE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (C-Term) antibodyCPE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 252-363) antibodyCPE 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 271-320) antibodyCPE 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Carboxypeptidase E (CPE) (AA 257-476) antibodyCPE 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS. -
说明
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Related Products: ABIN968545
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "Carboxypeptidase E is a regulated secretory pathway sorting receptor: genetic obliteration leads to endocrine disorders in Cpe(fat) mice." in: Cell, Vol. 88, Issue 1, pp. 73-83, (1997) (PubMed).
: "Intracellular misrouting and abnormal secretion of adrenocorticotropin and growth hormone in cpefat mice associated with a carboxypeptidase E mutation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5314-9, (1997) (PubMed).
: "The C-terminal region of carboxypeptidase E involved in membrane binding is distinct from the region involved with intracellular routing." in: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6077-83, (1996) (PubMed).
: "Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitro." in: The Biochemical journal, Vol. 267, Issue 2, pp. 517-25, (1990) (PubMed).
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: "Carboxypeptidase E is a regulated secretory pathway sorting receptor: genetic obliteration leads to endocrine disorders in Cpe(fat) mice." in: Cell, Vol. 88, Issue 1, pp. 73-83, (1997) (PubMed).
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- CPE (Carboxypeptidase E (CPE))
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别名
- Carboxypeptidase E
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背景
- Carboxypeptidase E (CPE), also known as carboxypeptidase H and enkephalin convertase, is found as both a membrane-bound and a soluble glycoprotein in neuroendocrine tissues and adrenal-gland chromaffin granules. The C-terminus forms an amphiphilic alpha-helix, suggesting that this region is responsible for the membrane-bound form. Evidence suggests the active form of CPE is located in the secretory vesicles. CPE appears to have several functions. It is an exopeptidase that cleaves neuropeptides with C-terminal basic amino acids, producing an active form of the peptide. It has also been proposed that membrane-bound CPE is a sorting receptor for regulated secretory pathway (RSP) proteins in the TGN pituitary Golgi and secretory granule membranes. RSP proteins primarily consist of hormones and neuropeptides. Mice that carry a mutation in the CPE gene Cpe[fat] display endocrine disorders such as obesity, infertility, and hyperproinsulinemia. Furthermore, the same endocrine disorders are observed in Cpe[fat] mice where the CPE gene has been effaced by antisense RNA.
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分子量
- 50 kDa
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途径
- Peptide Hormone Metabolism, Synaptic Membrane
抗原
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