Arginase, Liver (ARG1) (AA 53-207) 抗体

ABIN968127 产品详细信息, 供应商: Log in to see
抗原
  • SI:zC146F4.4 (novel protein with NUDIX domain)
  • si:ch211-146f4.3
  • argi1
  • AI
  • AI256583
  • Arg-1
  • PGIF
  • arginase 1
  • arginase
  • Arginase-1
  • arginase, liver
  • L-arginase
  • arg1
  • PGTG_16455
  • argi1
  • ARG1
  • Arg1
抗原表位
AA 53-207
70
42
18
18
10
7
7
7
5
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
适用
Fly (Calliphora), 小鼠, 大鼠
195
78
69
24
16
14
8
5
3
3
2
2
1
1
宿主
小鼠
157
48
41
10
4
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
13
13
10
10
8
7
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
201
119
44
43
36
21
20
13
5
4
3
2
2
2
1
1
1
1
1
1
选项
Supplier
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Supplier Product No.
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免疫原 Human Arginase I aa. 53-207
克隆位点 19-Arginase I
亚型 IgG1
交叉反应 小鼠, 大鼠, Fruit Fly (Drosophila melanogaster)
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
别名 Arginase I (ARG1 Antibody 摘要)
背景 Arginase converts arginine into urea plus ornithine, the final step in urea synthesis. Two different isoforms (I&II) have been isolated with approximately 60% homology at the nucleotide level. While type II is present in many tissues, Arginase I is expressed exclusively in liver. In cultured macrophages, as well as in vivo, Arginase I is induced with nitric oxide synthase (NOS) and the arginase I transactivator C/EBPbeta in response to lipopolysaccharide. This response occurs in a dose and time-dependent manner. While the mRNA for NOS appears as early as 2h after treatment, mRNA levels for arginase I peak after twelve hours of lipopolysaccharide treatment. Since the synthesis of nitric oxide by NOS requires arginine, the delayed induction of arginase I may be necessary for the regulation of NOS activity. This antibody is routinely tested by western blot analysis.
分子量 35 kDa
途径 Cellular Response to Molecule of Bacterial Origin
说明

Related Products: ABIN968543, ABIN967389

限制 仅限研究用
状态 Liquid
浓度 250 μg/mL
缓冲液 Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store undiluted at -20° C.
厂商提供的图像
Western Blotting (WB) image for anti-Arginase, Liver (ARG1) (AA 53-207) antibody (ABIN968127) Western blot analysis of Arginase I on a mouse liver lysate. Lane 1: 1:1000, lane 2: ...
Immunofluorescence (IF) image for anti-Arginase, Liver (ARG1) (AA 53-207) antibody (ABIN968127) Immunofluorescence staining of mouse macrophages.
有引用在: Morrison, Correll: "Activation of the stem cell-derived tyrosine kinase/RON receptor tyrosine kinase by macrophage-stimulating protein results in the induction of arginase activity in murine peritoneal macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 2, pp. 853-60, 2002 (PubMed).

Chang, Zoghi, Liao, Kuo: "The involvement of tyrosine kinases, cyclic AMP/protein kinase A, and p38 mitogen-activated protein kinase in IL-13-mediated arginase I induction in macrophages: its implications in IL-13-inhibited nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 4, pp. 2134-41, 2000 (PubMed).

Sonoki, Nagasaki, Gotoh, Takiguchi, Takeya, Matsuzaki, Mori: "Coinduction of nitric-oxide synthase and arginase I in cultured rat peritoneal macrophages and rat tissues in vivo by lipopolysaccharide." in: The Journal of biological chemistry, Vol. 272, Issue 6, pp. 3689-93, 1997 (PubMed).

Dizikes, Grody, Kern, Cederbaum: "Isolation of human liver arginase cDNA and demonstration of nonhomology between the two human arginase genes." in: Biochemical and biophysical research communications, Vol. 141, Issue 1, pp. 53-9, 1987 (PubMed).

Haraguchi, Takiguchi, Amaya, Kawamoto, Matsuda, Mori: "Molecular cloning and nucleotide sequence of cDNA for human liver arginase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 2, pp. 412-5, 1987 (PubMed).

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