Ezrin 抗体 (AA 391-515)
Quick Overview for Ezrin 抗体 (AA 391-515) (ABIN968057)
抗原
See all Ezrin (EZR) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 391-515
-
交叉反应
- 犬
-
产品特性
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
免疫原
- Human Ezrin aa. 391-515
-
亚型
- IgG1
-
-
-
-
说明
-
Related Products: ABIN968619, ABIN967389
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 250 μg/mL
-
缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store undiluted at -20° C.
-
-
-
Activation of the PKB/AKT pathway by ICAM-2." in: Immunity, Vol. 16, Issue 1, pp. 51-65, (2002) (PubMed).
: "Inhibition of RhoA by p120 catenin." in: Nature cell biology, Vol. 2, Issue 9, pp. 637-44, (2001) (PubMed).
: "Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes." in: The EMBO journal, Vol. 19, Issue 2, pp. 199-212, (2000) (PubMed).
: "Yes-associated protein 65 localizes p62(c-Yes) to the apical compartment of airway epithelia by association with EBP50." in: The Journal of cell biology, Vol. 147, Issue 4, pp. 879-90, (1999) (PubMed).
: "Ezrin oligomers are major cytoskeletal components of placental microvilli: a proposal for their involvement in cortical morphogenesis." in: The Journal of cell biology, Vol. 131, Issue 5, pp. 1231-42, (1996) (PubMed).
: "
-
Activation of the PKB/AKT pathway by ICAM-2." in: Immunity, Vol. 16, Issue 1, pp. 51-65, (2002) (PubMed).
-
- Ezrin (EZR)
-
别名
- Ezrin
-
背景
- First described as an 80kDa protein concentrated in the apical cytoskeletal region of intestinal brush border cells, ezrin is now recognized as a major substrate of protein tyrosine kinases, such as the epidermal growth factor (EGF) tyrosine kinase. Ezrin is expressed at high levels in intestine, kidney, and placenta. In placenta, ezrin is present as monomers and non-covalent oligomers in tight association with actin microfilaments. In the human epidermoid carcinoma cell line A431, microvilli-like structures appear within 30 seconds after the addition of EGF. These structures give way to membrane ruffles after 2-5 minutes, followed by cell rounding after 10-20 minutes. At the same time, ezrin is recruited into these structures and oligomers are formed following its tyrosine phosphorylation. It is thought that tyrosine phosphorylation triggers the formation of ezrin oligomers.
-
分子量
- 80 kDa
-
途径
- Maintenance of Protein Location
抗原
-