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Cyclin B1 抗体 (AA 14-168)

Name: Cyclin B1 抗体 (AA 14-168)
SKU: ABIN967834
Availability: OutOfStock

This anti-Cyclin B1 antibody is a 小鼠单克隆 antibody detecting Cyclin B1 in WB, IF, IP 和 IHC (f). Suitable for 人和大鼠. This Primary Antibody has been cited in 5+ publications.

Western blot analysis of cyclin B on a Jurkat cell lysate. (Cyclin B1 抗体 (AA 14-168))

Immunofluorescence staining of SKN cells (human neuroblastoma). (Cyclin B1 抗体 (AA 14-168))

Image no. 3 for anti-Cyclin B1 (CCNB1) (AA 14-168) antibody (ABIN967834) (Cyclin B1 抗体 (AA 14-168))

3 images

(5 references)

产品编号 ABIN967834

发货至: 中国

Datasheet as PDF

Quick Overview for Cyclin B1 抗体 (AA 14-168) (ABIN967834)

抗原

See all Cyclin B1 (CCNB1) 抗体

Cyclin B1 (CCNB1)

适用

人, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This Cyclin B1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

18-Cyclin B

Discover our top product CCNB1 Primary Antibody

抗原表位
30

22

16

16

8

8

7

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 14-168

交叉反应

大鼠

产品特性

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

免疫原

Human Cyclin B aa. 14-168

亚型

IgG1
anti-Cyclin B1 (CCNB1) (C-Term) antibody
CCNB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B1 (CCNB1) antibody
CCNB1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B1 (CCNB1) antibody
CCNB1 适用: 人, 小鼠 IHC, StM 宿主: 小鼠 Monoclonal CCNB1-1098 unconjugated

anti-Cyclin B1 (CCNB1) (C-Term) antibody
CCNB1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B1 (CCNB1) (AA 271-433) antibody
CCNB1 适用: 人, 小鼠, 大鼠, Cow, 小鸡 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B1 (CCNB1) (AA 1-433) antibody
CCNB1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B1 (CCNB1) (AA 1-90) antibody
CCNB1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1C8 unconjugated

anti-Cyclin B1 (CCNB1) (pSer126) antibody
CCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B1 (CCNB1) (Internal Region) antibody
CCNB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin B1 (CCNB1) antibody
KO Validated CCNB1 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

说明

Related Products: ABIN968537, ABIN967389

限制

仅限研究用
状态

Liquid

浓度

250 μg/mL

缓冲液

Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store undiluted at -20° C.
Miyoshi, Rosner, Nozawa, Byrd, Morgan, Landesman-Bollag, Xu, Seldin, Schmidt, Taketo, Robinson, Cardiff, Hennighausen: "Activation of different Wnt/beta-catenin signaling components in mammary epithelium induces transdifferentiation and the formation of pilar tumors." in: Oncogene, Vol. 21, Issue 36, pp. 5548-56, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Topper, Campbell, Tugendreich, Daum, Burke, Hieter, Gorbsky: "The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis." in: Cell cycle (Georgetown, Tex.), Vol. 1, Issue 4, pp. 282-92, (2002) (PubMed).

Takasaki, Kogure, Takeuchi, Kaneda, Yano, Hirokawa, Hirose, Shirai, Hashimoto: "Reactivity of anti-proliferating cell nuclear antigen (PCNA) murine monoclonal antibodies and human autoantibodies to the PCNA multiprotein complexes involved in cell proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 7, pp. 4780-7, (2001) (PubMed).

Mailand, Falck, Lukas, Syljuâsen, Welcker, Bartek, Lukas: "Rapid destruction of human Cdc25A in response to DNA damage." in: Science (New York, N.Y.), Vol. 288, Issue 5470, pp. 1425-9, (2000) (PubMed).
抗原

Cyclin B1 (CCNB1)

别名

Cyclin B

背景

The B-type cyclins serve as subunits of the major mitotic protein kinase in many different organisms. In mammals, Cyclin B is a subunit for the cdc2 (Cdk1) protein kinase and is synthesized during late S and G2 phases. Cyclin B associates with inactive cdc2 and facilitates phosphorylation of cdc2 at Thr-14 and Tyr-15. This phosphorylation maintains the inactive state until the end of G2. The inactive Cyclin B-cdc2 complex continues to accumulate in the cytoplasm until the CDC25 phosphatase dephosphorylates Thr-14 and Tyr-15, rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B becomes degraded. This degradation process appears to be ubiquitin-dependent and is necessary for the cell to exit mitosis. Also, the conserved domain in B-type cyclins, known as the P box, is required for CDC25 activation. This antibody is routinely tested by western blot analysis.

分子量

62 kDa

途径

Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase