RAP2A 抗体 (AA 1-183)
(5 references)Quick Overview for RAP2A 抗体 (AA 1-183) (ABIN967831)
抗原
See all RAP2A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-183
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交叉反应
- 小鸡, 犬, Frog, 小鼠, 大鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human Rap2 aa. 1-183
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亚型
- IgG2a
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anti-RAP2A, Member of RAS Oncogene Family (RAP2A) (AA 1-183) antibody
RAP2A 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-RAP2A, Member of RAS Oncogene Family (RAP2A) (AA 140-190) antibodyRAP2A 适用: 人, 小鼠, 犬, 马, 非洲爪蟾, 猴, 小鸡, Opossum WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAP2A, Member of RAS Oncogene Family (RAP2A) (AA 16-38) antibodyRAP2A 适用: 小鼠 WB 宿主: 兔 Polyclonal RB53677 unconjugated
anti-RAP2A, Member of RAS Oncogene Family (RAP2A) (AA 124-159) antibodyRAP2A 适用: 人 WB 宿主: 兔 Polyclonal RB53398 unconjugated
anti-RAP2A, Member of RAS Oncogene Family (RAP2A) (N-Term) antibodyRAP2A 适用: 人, 小鼠, 大鼠, 犬, 马, Cow, 斑马鱼, 绵羊, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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说明
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Related Products: ABIN968533, ABIN967389
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20° C.
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: "Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade." in: Molecular and cellular biology, Vol. 20, Issue 16, pp. 6074-83, (2000) (PubMed).
: "Costimulation through CD28 suppresses T cell receptor-dependent activation of the Ras-like small GTPase Rap1 in human T lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4944-9, (1998) (PubMed).
: "Regulation of the GTPase activity of the ras-related rap2 protein." in: Biochemical and biophysical research communications, Vol. 189, Issue 1, pp. 455-64, (1992) (PubMed).
: "RAP2B: a RAS-related GTP-binding protein from platelets." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 17, pp. 6527-31, (1990) (PubMed).
: "Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the 'effector' region." in: Oncogene, Vol. 3, Issue 2, pp. 201-4, (1988) (PubMed).
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: "Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade." in: Molecular and cellular biology, Vol. 20, Issue 16, pp. 6074-83, (2000) (PubMed).
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- RAP2A (RAP2A, Member of RAS Oncogene Family (RAP2A))
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别名
- Rap2
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背景
- Rap2 is a member of the Ras superfamily of low molecular weight GTP/GDP binding proteins. The Rap proteins are 50% homologous in sequence to p21ras. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Conversion between these two forms is regulated by Rap-GTPase activating protein (Rap-GAP) and a Rap-GDP dissociation stimulator (GDS). Since Ras and Rap have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic functions. Rap2 proteins are about 60% identical to Rap1 proteins and Rap2A and Rap2B show 90% amino acid identity, differing mainly at the carboxy-terminus. Unlike Rap1, the Rap2 proteins cannot compete with Ras for interaction with Ras-GAP, nor are they substrates for PKA. It follows that the intrinsic GTPase activity of Rap2A is not stimulated by Ras-GAP, however a distinct activator (Rap-GAP) has been identified. Both Rap2 proteins show posttranslational modifications: Rap2B is geranylgeranylated and Rap2A is the first non-Ras member of the Ras superfamily observed to be farnesylated. This antibody is routinely tested by western blot analysis.
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分子量
- 21 kDa
抗原
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